Anti-Human E-selectin/CD62E Rabbit Recombinant Antibody

E-selectin/CD62E Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241767H6

Cat no : 98189-1-RR

Synonyms

CD62E, E-selectin, ELAM 1, ELAM, E selectin

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Validation Data Gallery

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  FC experiment of HUVEC using 98189-1-RR

  1x10^6 untreated or TNF alpha treated HUVEC cells were surface stained with 0.25 ug Anti-Human E-selectin/CD62E Rabbit Recombinant Antibody (98189-1-RR, Clone: 241767H6) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  FC experiment of HUVEC using 98189-1-RR

  1x10^6 TNF alpha treated HUVEC cells were surface stained with 0.25 ug Anti-Human E-selectin/CD62E Rabbit Recombinant Antibody (98189-1-RR, Clone: 241767H6) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated HUVEC cells were surface stained with 0.25 ug Anti-Human E-selectin/CD62E Rabbit Recombinant Antibody (98189-1-RR, Clone: 241767H6) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (black, dashed). Cells were not fixed.

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Tested Applications

• Positive FC detected in: TNF alpha treated HUVEC cells

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98189-1-RR targets E-selectin/CD62E in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: selectin E
• Calculated Molecular Weight: 610 aa, 67 kDa
• GenBank Accession Number: BC142711
• Gene Symbol: CD62E
• Gene ID (NCBI): 6401
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

E-selectin, also known as CD62E or ELAM-1, is a member of the selectin family of adhesion molecules that also includes L-selectin and P-selectin. E-selectin is an inducible endothelial cell surface molecule. Its expression on endothelial cells is transcriptionally upregulated by various proinflammatory substances such as IL-1, TNFα and lipopolysaccharide (LPS). Besides, it can be secreted by endothelial cells, and can be detected in a soluble form (sE-selectin) in serum. During inflammation, E-selectin plays an important part in recruiting leukocytes to the site of injury. (PMID: 2827173; 1382417; 8943958)