DLAT Recombinant antibody, PBS Only (Capture)

DLAT Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240713C12

Cat no : 83654-3-PBS

Synonyms

PDC-E2, ODP2, EC:2.3.1.12, DLTA, Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial

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Validation Data Gallery

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  WB analysis using 83654-3-RR (same clone as 83654-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83654-3-RR (DLAT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83654-3-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00635-4

  Sandwich ELISA standard curve of MP00635-4, Human DLAT Recombinant Matched Antibody Pair - PBS only. 83654-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag22831. 83654-5-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL

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  Sample test of MP00635-4

  The mean DLAT concentration was determined to be 115.60 ng/mL in HepG2 cell extract based on a 1.2 mg/mL extract load and 96.07 ng/mL in HEK-293 cell extract based on a 2.3 mg/mL extract load.

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  IF Staining of HepG2 using 83654-3-RR (same clone as 83654-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DLAT antibody (83654-3-RR, Clone: 240713C12 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83654-3-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 83654-3-RR (same clone as 83654-3-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug DLAT Recombinant Antibody (83654-3-RR, Clone:240713C12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83654-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00635-2

  Cytometric bead array standard curve of MP00635-2, DLAT Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83654-3-PBS. Detection antibody: 83654-1-PBS. Standard: Ag22831. Range: 0.313-40 ng/mL.

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  Cytometric bead array standard curve of MP00635-3

  Cytometric bead array standard curve of MP00635-3, DLAT Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83654-3-PBS. Detection antibody: 83654-2-PBS. Standard: Ag22831. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 83654-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83654-3-RR against Human DLAT were performed. The affinity constant is 0.17 nM.

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Product Information

83654-3-PBS targets DLAT as part of a matched antibody pair:

MP00635-2: 83654-3-PBS capture and 83654-1-PBS detection (validated in Cytometric bead array)

MP00635-3: 83654-3-PBS capture and 83654-2-PBS detection (validated in Cytometric bead array)

MP00635-4: 83654-3-PBS capture and 83654-5-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: DLAT fusion protein Ag22831
• Full Name: dihydrolipoamide S-acetyltransferase
• Calculated Molecular Weight: 647 aa, 70 kDa
• Observed Molecular Weight: 69-70 kDa
• GenBank Accession Number: BC039084
• Gene Symbol: DLAT
• Gene ID (NCBI): 1737
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.