DLAT Recombinant antibody

DLAT Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240713C12

Cat no : 83654-3-RR

Synonyms

PDC-E2, ODP2, EC:2.3.1.12, DLTA, Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial

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Validation Data Gallery

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  WB analysis using 83654-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83654-3-RR (DLAT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 83654-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DLAT antibody (83654-3-RR, Clone: 240713C12 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  FC experiment of HepG2 using 83654-3-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug DLAT Recombinant Antibody (83654-3-RR, Clone:240713C12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83654-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83654-3-RR against Human DLAT were performed. The affinity constant is 0.17 nM.

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells, LNCaP cells, mouse brain tissue, rat brain tissue
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83654-3-RR is DLAT Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: DLAT fusion protein Ag22831
• Full Name: dihydrolipoamide S-acetyltransferase
• Calculated Molecular Weight: 647 aa, 70 kDa
• Observed Molecular Weight: 69-70 kDa
• GenBank Accession Number: BC039084
• Gene Symbol: DLAT
• Gene ID (NCBI): 1737
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.