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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated DEK Monoclonal antibody

DEK Monoclonal Antibody for WB, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

5E3A6

Cat no : CL594-66194

Synonyms

D6S231E, 5E3A6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CoraLite®594-conjugated DEK Monoclonal antibody (CL594-66194)

    WB analysis using CL594-66194

    Various lysates were subjected to SDS PAGE followed by western blot with CL594-66194 (DEK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated DEK Monoclonal antibody (CL594-66194)

    FC experiment of HeLa using CL594-66194

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human DEK (CL594-66194, Clone:5E3A6) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells, HepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66194 targets DEK in WB, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen DEK fusion protein Ag8225
Full Name DEK oncogene
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC035259
Gene Symbol DEK
Gene ID (NCBI) 7913
RRIDAB_2919963
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Dek oncogene(DEK) is first recognized in a fusion with CAN nucleoporin protein, and it's a also autoantigen in patients with pquciarticular onset juvenile rheumatoid arthritis. As a site-specific binding protein, DEK may involve in transcriptional regulation and signal transdcution. Analysis by agarose gel celctrophoresis, DEK showed to reduce the efficiency with which chromation is replicated. It's one of the first components of the splicing complex, which remains associate with spliced exons dependent on prior splicing of pre-mRNA.

Protocols

Product Specific Protocols
WB protocol for CL594 DEK antibody CL594-66194Download protocol
Standard Protocols
Click here to view our Standard Protocols