DEK Monoclonal antibody

• Featured Product
• KD/KO Validated

DEK Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

CloneNo.

5E3A6

Cat no : 66194-1-Ig

Synonyms

D6S231E, 5E3A6

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Validation Data Gallery

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  WB analysis using 66194-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66194-1-Ig (DEK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66194-1-Ig

  WB result of DEK antibody (66194-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DEK transfected HEK-293 cells.

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  IF Staining of HepG2 using 66194-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DEK antibody (66194-1-Ig, Clone: 5E3A6 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, Raji cells, Ramos cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

66194-1-Ig targets DEK in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: DEK fusion protein Ag8225
• Full Name: DEK oncogene
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 40-50 kDa
• GenBank Accession Number: BC035259
• Gene Symbol: DEK
• Gene ID (NCBI): 7913
• RRID: AB_2881588
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Dek oncogene(DEK) is first recognized in a fusion with CAN nucleoporin protein, and it's a also autoantigen in patients with pquciarticular onset juvenile rheumatoid arthritis. As a site-specific binding protein, DEK may involve in transcriptional regulation and signal transdcution. Analysis by agarose gel celctrophoresis, DEK showed to reduce the efficiency with which chromation is replicated. It's one of the first components of the splicing complex, which remains associate with spliced exons dependent on prior splicing of pre-mRNA.

Publications

Species	Application	Title
mouse	WB	J Exp Med; Nuclear DEK preserves hematopoietic stem cells potential via NCoR1/HDAC3-Akt1/2-mTOR axis.; Authors - Zhe Chen
human	WB,IF	Biochem J; DEK terminates diapause by activation of quiescent cells in the crustacean Artemia.; Authors - Wen-Huan Jia ; KD Validated
	IHC	Mol Med Rep; Overexpression of DEK gene is correlated with poor prognosis in hepatocellular carcinoma.; Authors - Huo-Chun Yi