CoraLite®555-conjugated DDX5 Monoclonal antibody

DDX5 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

2D3D2

Cat no : CL555-67025

Synonyms

HLR1, HELR, G17P1, EC:3.6.4.13, DDX5,p68

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Validation Data Gallery

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  IF Staining of HepG2 using CL555-67025

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®555 DDX5 antibody (CL555-67025, Clone: 2D3D2 ) at dilution of 1:200.

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  FC experiment of HeLa using CL555-67025

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human DDX5 (CL555-67025, Clone:2D3D2) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL555-67025 targets DDX5 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: DDX5 fusion protein Ag24821
• Full Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 5
• Observed Molecular Weight: 69 kDa
• GenBank Accession Number: NM_004396
• Gene Symbol: DDX5
• Gene ID (NCBI): 1655
• RRID: AB_2919698
• Conjugate: CoraLite®555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 557 nm / 570 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Related Products

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011