DDX5 Monoclonal antibody

DDX5 Monoclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

4

CloneNo.

2D3D2

Cat no : 67025-1-Ig

Synonyms

DDX5, DEAD box protein 5, DKFZp434E109, DKFZp686J01190, G17P1, HELR, HLR1, HUMP68, p68, RNA helicase p68

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Validation Data Gallery

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  WB analysis using 67025-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67025-1-Ig (DDX5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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  WB analysis using 67025-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67025-1-Ig (DDX5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67025-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67025-1-Ig (DDX5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 67025-1-Ig

  IP result of anti-DDX5 (IP:67025-1-Ig, 5ug; Detection:67025-1-Ig 1:1000) with HeLa cells lysate 1600 ug.

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  IHC staining of human colon cancer using 67025-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67025-1-Ig (DDX5 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 67025-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67025-1-Ig (DDX5 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 67025-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DDX5 antibody (67025-1-Ig, Clone: 2D3D2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

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  IF Staining of HepG2 using 67025-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67025-1-Ig (DDX5 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: NCI-H1299 cells, A431 cells, HSC-T6 cells, HeLa cells, HEK-293 cell, HepG2 cells, HT-29 cells, NIH/3T3 cells, 4T1 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

67025-1-Ig targets DDX5 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: DDX5 fusion protein Ag24821
• Full Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 5
• Observed Molecular Weight: 69 kDa
• GenBank Accession Number: NM_004396
• Gene Symbol: DDX5
• Gene ID (NCBI): 1655
• RRID: AB_2882340
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DDX5 or p68, a member of DEAD/H BOX, which has a characteristic DEAD box, is a proliferation-associated nuclear antigen. DDX5 involves in the alternative regulation of pre-mRNA splicing, act as a RNA helicase to increase tau exon10 inclusion in a RBM4-dependent manner. It's also a transcription coactivator for extrogen receptor ESR1 and androgen receptor AR. Once synergized with DDX17 and SRA1 RNA, DDX5 activated MYOD1 transcription activity and involved in skeletal muscle differentiation.

Publications

Species	Application	Title
human	CoIP	Theranostics; LncRNA PRADX-mediated recruitment of PRC2/DDX5 complex suppresses UBXN1 expression and activates NF-κB activity, promoting tumorigenesis.; Authors - Yansheng Li
human	WB,IP	Cancer Biol Ther; VAMP8 suppresses the metastasis via DDX5/β-catenin signal pathway in osteosarcoma; Authors - Shuo Yang
human	WB,IP	Virol J; DEAD box RNA helicase 5 is a new pro-viral host factor for Sindbis virus infection; Authors - Mélanie Messmer
human	WB	J Am Chem Soc; Polymerase η Recruits DHX9 Helicase to Promote Replication across Guanine Quadruplex Structures; Authors - Feng Tang