CoraLite® Plus 488-conjugated DDX39A Recombinant antibody

• Featured Product
• KD/KO Validated

DDX39A Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230375F9

Cat no : CL488-83083-5

Synonyms

DDX39, DDXL, BAT1L, BAT1, ATP-dependent RNA helicase DDX39A

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-83083-5

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 DDX39A antibody (CL488-83083-5, Clone: 230375F9 ) at dilution of 1:200.

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  FC experiment of Jurkat using CL488-83083-5

  1x10^6 Jurkat cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Ddx39a Recombinant Antibody (CL488-83083-5, Clone:230375F9)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: Jurkat cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-83083-5 targets DDX39A in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: DDX39A fusion protein Ag2311
• Full Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 39
• Calculated Molecular Weight: 427 aa, 49 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC032128
• Gene Symbol: DDX39
• Gene ID (NCBI): 10212
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

DDX39A, also named the BAT1 protein, contains the nine conserved motifs that characterize the DEAD-box family of RNA-binding proteins. The family includes proteins found in all eukaryotic cell types, with considerable divergence in the sequences lying between the conserved motifs. Some of the motifs were known before the definition of the family and are responsible for binding to mRNA or ATP, or possess ATPase activity. Phylogenetic analyses have grouped BAT1 with the defining member of the DEAD-box family, eIF-4. This is a translation initiation factor required for the dissociation of stem/loop structures in mRNA at the ribosomes.