DDX39A Recombinant antibody

• Featured Product
• KD/KO Validated

DDX39A Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230375F9

Cat no : 83083-5-RR

Synonyms

DDX39, DDXL, BAT1L, BAT1, ATP-dependent RNA helicase DDX39A

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Validation Data Gallery

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  WB analysis using 83083-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83083-5-RR (DDX39 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 83083-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83083-5-RR (DDX39A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 83083-5-RR

  WB result of DDX39A antibody (83083-5-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDX39A transfected HEK-293 cells.

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  IHC staining of mouse kidney using 83083-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83083-5-RR (DDX39A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 83083-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DDX39 antibody (83083-5-RR, Clone: 230375F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

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  FC experiment of Jurkat using 83083-5-RR

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug Anti-Human DDX39A (83083-5-RR, Clone:230375F9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  Affinity and Kinetic Characterization of 83083-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83083-5-RR against Human DDX39A were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: Jurkat cells, HEK-293 cells, mouse kidney tissue, HeLa cells, HepG2 cells, Raji cells, A431 cells
• Positive IHC detected in: mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83083-5-RR targets DDX39A in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: DDX39A fusion protein Ag2311
• Full Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 39
• Calculated Molecular Weight: 427 aa, 49 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC032128
• Gene Symbol: DDX39
• Gene ID (NCBI): 10212
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DDX39A, also named the BAT1 protein, contains the nine conserved motifs that characterize the DEAD-box family of RNA-binding proteins. The family includes proteins found in all eukaryotic cell types, with considerable divergence in the sequences lying between the conserved motifs. Some of the motifs were known before the definition of the family and are responsible for binding to mRNA or ATP, or possess ATPase activity. Phylogenetic analyses have grouped BAT1 with the defining member of the DEAD-box family, eIF-4. This is a translation initiation factor required for the dissociation of stem/loop structures in mRNA at the ribosomes.