CoraLite® Plus 647-conjugated Cytokeratin 6A Polyclonal antibody
Cytokeratin 6A Polyclonal Antibody for IF, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite® Plus 647 Fluorescent Dye
Cat no : CL647-10590
Synonyms
Validation Data Gallery
Tested Applications
Positive IF/ICC detected in | A431 cells |
Positive FC (Intra) detected in | A431 cells |
Positive FC detected in | A431 cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL647-10590 targets Cytokeratin 6A in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Cytokeratin 6A fusion protein Ag0882 |
Full Name | keratin 6A |
Calculated Molecular Weight | 60 kDa |
Observed Molecular Weight | 56 kDa |
GenBank Accession Number | BC008807 |
Gene Symbol | Cytokeratin 6A |
Gene ID (NCBI) | 3853 |
Conjugate | CoraLite® Plus 647 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 6 is a type II keratin. It is used as marker for epidermal hyperproliferation and differentiation. Three are three keratin 6 isoforms: ketatin 6A,6B and 6C. They share more than 99% identical DNA sequence.
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 647 Cytokeratin 6A antibody CL647-10590 | Download protocol |
FC protocol for CL Plus 647 Cytokeratin 6A antibody CL647-10590 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |