Cytokeratin 6A Polyclonal antibody

Cytokeratin 6A Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

22

Cat no : 10590-1-AP

Synonyms

Cytokeratin 6, KRT6A, CK-6D, CK-6A, CK6A

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Validation Data Gallery

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  WB analysis of A431 using 10590-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skin using 10590-1-AP

  rat skin tissue were subjected to SDS PAGE followed by western blot with 10590-1-AP (Cytokeratin 6A antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-251 using 10590-1-AP

  U-251 cells were subjected to SDS PAGE followed by western blot with 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10590-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10590-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10590-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 10590-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human oesophagus cancer using 10590-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 10590-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 10590-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10590-1-AP (Cytokeratin 6A antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human lung cancer using 10590-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10590-1-AP (Cytokeratin 6A antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human skin using 10590-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 10590-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 10590-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10590-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 10590-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cytokeratin 6A antibody (10590-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A431 using 10590-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using 10590-1-AP (Cytokeratin 6A antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 10590-1-AP

  Immunofluorescent analysis of Hela cells, using KRT6A antibody 10590-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of A431 using 10590-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human Cytokeratin 6A (10590-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: A431 cells, MCF-7 cells, HeLa cells, A375 cells, rat skin tissue, U-251 cells
• Positive IHC detected in: human oesophagus cancer tissue, human breast cancer tissue, human skin tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells, HeLa cells
• Positive FC (Intra) detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:10000-1:50000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 11 publications below
• IHC: See 7 publications below
• IF: See 8 publications below

Product Information

10590-1-AP targets Cytokeratin 6A in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cytokeratin 6A fusion protein Ag0882
• Full Name: keratin 6A
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC008807
• Gene Symbol: Cytokeratin 6A
• Gene ID (NCBI): 3853
• RRID: AB_2134306
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 6 is a type II keratin. It is used as marker for epidermal hyperproliferation and differentiation. Three are three keratin 6 isoforms: ketatin 6A,6B and 6C. They share more than 99% identical DNA sequence.

Publications

Species	Application	Title
mouse	IF	Immunity; Excessive Polyamine Generation in Keratinocytes Promotes Self-RNA Sensing by Dendritic Cells in Psoriasis.; Authors - Fangzhou Lou
		Cell Rep; Keratinocyte FABP5-VCP complex mediates recruitment of neutrophils in psoriasis; Authors - Jiaqing Hao
human,mouse	IF	Cell Rep; Collagen 1-mediated CXCL1 secretion in tumor cells activates fibroblasts to promote radioresistance of esophageal cancer; Authors - Xinyu Yang
mouse	WB	Phytother Res; Epigallocatechin-3-gallate promotes wound healing response in diabetic mice by activating keratinocytes and promoting re-epithelialization; Authors - Yongling Ning
human,mouse	WB,IHC,IF	J Cell Mol Med; Ozone therapy promotes the differentiation of basal keratinocytes via increasing Tp63-mediated transcription of KRT10 to improve psoriasis.; Authors - Lihua Gao
human	WB	J Eur Acad Dermatol Venereol; Differentially expressed proteins identified by TMT proteomics analysis in children with verrucous epidermal naevi.; Authors - T Yuan