CoraLite®594-conjugated Cytokeratin 18 Monoclonal antibody

Cytokeratin 18 Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1G11C4

Cat no : CL594-66187

Synonyms

CK 18, CYK18, Cytokeratin 18, K18, keratin 18, KRT18

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Validation Data Gallery

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  IF Staining of HeLa using CL594-66187

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL594-66187 (Cytokeratin 18 antibody) at dilution of 1:100.

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  IF Staining of HepG2 using CL594-66187

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-66187 (Cytokeratin 18 antibody) at dilution of 1:200.

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  FC experiment of HeLa using CL594-66187

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human KRT18 (CL594-66187, Clone:1G11C4) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: HeLa cells, HepG2 cells
• Positive FC (Intra) detected in: HeLa cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66187 targets Cytokeratin 18 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Cytokeratin 18 fusion protein Ag1260
• Full Name: keratin 18
• Calculated Molecular Weight: 48 kDa
• GenBank Accession Number: BC000180
• Gene Symbol: Cytokeratin 18
• Gene ID (NCBI): 3875
• RRID: AB_2883529
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT18, also named as CYK18, PIG46 and K18, is the most commonly found members of the intermediate filament gene family. KRT18 is a type I keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. The antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C