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CoraLite®555-conjugated Cytokeratin 18 Monoclonal antibody

Cytokeratin 18 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

1G11C4

Cat no : CL555-66187

Synonyms

CK 18, CYK18, Cytokeratin 18, K18, keratin 18, KRT18

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®555-conjugated Cytokeratin 18 Monoclonal (CL555-66187)

    IF Staining of HepG2 using CL555-66187

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®555 Cytokeratin 18 antibody (CL555-66187, Clone: 1G11C4 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®555-conjugated Cytokeratin 18 Monoclonal (CL555-66187)

    FC experiment of HeLa using CL555-66187

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite®555 Anti-Human Cytokeratin 18 (CL555-66187, Clone:1G11C4) (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-66187 targets Cytokeratin 18 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Cytokeratin 18 fusion protein Ag1260
Full Name keratin 18
Calculated Molecular Weight 48 kDa
GenBank Accession NumberBC000180
Gene Symbol Cytokeratin 18
Gene ID (NCBI) 3875
RRIDAB_2919677
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT18, also named as CYK18, PIG46 and K18, is the most commonly found members of the intermediate filament gene family. KRT18 is a type I keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas.

Protocols

Product Specific Protocols
IF protocol for CL555 Cytokeratin 18 antibody CL555-66187Download protocol
FC protocol for CL555 Cytokeratin 18 antibody CL555-66187Download protocol
Standard Protocols
Click here to view our Standard Protocols