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CoraLite® Plus 488-conjugated Cyclin H Monoclonal antibody

Cyclin H Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2C5G11

Cat no : CL488-67065

Synonyms

CCNH, 2C5G11, p34, MO15-associated protein, MO15 associated protein

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using CoraLite® Plus 488-conjugated Cyclin H Monoclonal (CL488-67065)

    IF Staining of PC-3 using CL488-67065

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using CoraLite® Plus 488 Cyclin H antibody (CL488-67065, Clone: 2C5G11 ) at dilution of 1:200, CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of PC-3 cells using CoraLite® Plus 488-conjugated Cyclin H Monoclonal (CL488-67065)

    FC experiment of PC-3 using CL488-67065

    1X10^6 PC-3 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human Cyclin H (CL488-67065, Clone:2C5G11) (red), or 0.8 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive IF/ICC detected inPC-3 cells
Positive FC (Intra) detected inPC-3 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67065 targets Cyclin H in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Cyclin H fusion protein Ag28556
Full Name cyclin H
Calculated Molecular Weight 38 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC005280
Gene Symbol Cyclin H
Gene ID (NCBI) 902
RRIDAB_2919416
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Cyclin H antibody CL488-67065Download protocol
Standard Protocols
Click here to view our Standard Protocols