ChromoTek Nano-Secondary® alpaca anti-human IgG, recombinant VHH, for 2x Cys conjugation [CTK0117]

Monovalent, recombinant secondary single domain antibody to rabbit IgG: alpaca monoclonal Nanobody, Fab-specific, for cysteine conjugation with thiol-reactive reagents, e.g. maleimides

Applications

IF, WB, FC

Reactivity

Human

Host

Alpaca

Conjugate

2x Cys

Cat no : shuGCys2-2

Synonyms

Alpaca single domain antibody, VHH, Nanobody, binding domain of single domain antibody, Nano-antibody

---

Validation Data Gallery

• 

  The alpaca anti-human IgG Fc-specific recombinant VHH [CTK0117] has a very high affinity (KD <10 pM) for human IgG primary antibody.

• 

  The affinity of anti-human IgG recombinant VHH towards human IgG1 was determined using biolayer interferometry (BLI). Biotinylated, monoclonal human IgG1 was immobilized on Streptavidin biosensors and assayed with 1.5 to 25 nM of anti-human IgG recombinant VHH. Fit indicates a 1:1 binding model fitted to the data. 

---

Product Information

Monovalent, recombinant secondary single domain antibody to rabbit IgG: alpaca monoclonal Nanobody, Fab-specific, for cysteine conjugation with thiol-reactive reagents, e.g. maleimides

• Description: Monovalent, recombinant secondary single domain antibody to human ; IgG: alpaca monoclonal Nanobody, Fc-specific, for 2x Cys conjugation
• Applications: IF, WB, FC
• Host: Alpaca
• Reactivity: Reactivity: human IgG1, IgG2, IgG3, IgG4, Fc-fragment of human IgG, including wt, PGLALA and PALALA mutations. Slight reactivity to macaque serum. No cross-reactivity: human IgA/IgM/IgE, serum proteins from goat, guinea pig, mouse rabbit, rat, sheep.
• Specificity/Target: Fc-fragment of human IgG
• Conjugate: 2x Cys
• Molecular weight: 14.5 kDa
• Extinction coefficient (280 nm): 29700 L·Mol^-1·cm^-1
• Type: Secondary Nanobody
• Format: Alpaca single domain antibody, monovalent
• Cross-reactivity: No cross-reactivity to human IgA/IgM/IgE, and goat, guinea pig, mouse, ; rabbit, rat, sheep serum. Shows slight cross-reactivity to macaque serum.
• Immunogen: Purified human IgG
• Clonality: Monoclonal Nanobody
• Clone: CTK0117
• RRID: AB_3075306
• Affinity (KD): < 10 pM
• Storage Buffer: 10 mM HEPES pH 7.0, 500 mM NaCl, 1 mM TCEP, Preservative: 0.09 % Sodium azide
• Storage Condition: Shipped on dry ice. Store at -80°C. Avoid freeze-thaw cycles. Stable for 1 year at -80°C.
• Size: 500 µg