ChromoTek Nano-Secondary® alpaca anti-human IgG, recombinant VHH, CoraLite® Plus 750 [CTK0117]

Nano-Secondary® alpaca anti-human IgG, recombinant VHH, is a human IgG-specific (IgG1, IgG2, IgG3, IgG4) antibody. This secondary antibody product consists of a Nanobody that binds to the Fc-domain of human IgG with high affinity & specificity.

Applications

IF, FC

Conjugate

CoraLite® Plus 750

Host

Alpaca

Reactivity

human IgG

Cat no : shuGCL750-2

Synonyms

Alpaca single domain antibody, VHH, Nanobody, binding domain of single domain antibody, Nano-antibody

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Validation Data Gallery

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  Live A431 cells were incubated with anti-human EGFR (Cetuximab biosimilar) followed by Nano-Secondary® alpaca anti-human IgG, recombinant VHH, CoraLite® Plus 750 [CTK0117] (shuGCL750-2, gray). Cells were fixed and nuclei were stained with DAPI (blue). Epifluorescence images were acquired with a 40x objective and post-processed.

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  Immunofluorescence analysis of transgenic HeLa cells stably expressing H2B-myc. PFA-fixed cells were stained with anti-c-myc tag antibody and Nano-Secondary® alpaca anti-human IgG, recombinant VHH, CoraLite® Plus 750 [CTK0117] (shuGCL750-2, magenta).​

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  Schematic representation of anti-human IgG Nano-Secondary binding to its target. Human IgG primary antibody (blue) is bound with two identical monoclonal human IgG Fc- specific Nanobody molecules (green). In total, 4 fluorophores (red stars) label the human IgG primary antibody.

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Product Information

Nano-Secondary® alpaca anti-human IgG, recombinant VHH, is a human IgG-specific (IgG1, IgG2, IgG3, IgG4) antibody. This secondary antibody product consists of a Nanobody that binds to the Fc-domain of human IgG with high affinity & specificity.

• Applications: IF, FC
• Description: Nano-Secondary® alpaca anti-human IgG, Fc-specific recombinant VHH reagent uses a novel class of human IgG-specific antibodies. This secondary antibody product consists of a Nanobody that binds to human IgGs (IgG1, IgG2, IgG3, IgG4) with high affinity & specificity. Due to their small size, Nano-Secondary reagents provide enhanced image resolution. No cross-reactivity against serum proteins from other species apart from slight reactivity to macaque serum, details see below.
• Host: Alpaca
• Species Reactivity: Reactivity: human IgG1, IgG2, IgG3, IgG4, Fc-fragment of human IgG, including wt, PGLALA and PALALA mutations. Slight reactivity to macaque serum.; No cross-reactivity: human IgA/IgM/IgE, serum proteins from goat, guinea pig, mouse rabbit, rat, sheep.
• Physical State: Liquid
• Conjugate: CoraLite® Plus 750
• Suggested Dilution: Immunofluorescence 1:500 - 1:1000; Flow cytometry: 1µL per 1x10^6 cells
• Purification Method: Recombinant expression, affinity purification IMAC
• Type: Nanobody
• Class: Recombinant
• Affinity (KD): < 10 pM
• Storage Buffer: 10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % Sodium azide
• Storage Condition: Shipped at ambient temperature. Store at +4°C short term or -20°C long term. Stable for 1 year at -20°C.
• RRID: AB_3075305
• Note: This product is for research use only, not for diagnostic or therapeutic use