CYP3A4 Monoclonal antibody, PBS Only

CYP3A4 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F11C4

Cat no : 67110-1-PBS

Synonyms

Albendazole monooxygenase, Albendazole sulfoxidase, CP33, CP34, CYP3A, CYP3A3, CYP3A4, CYPIIIA3, CYPIIIA4, Cytochrome P450 3A3, Cytochrome P450 3A4, Cytochrome P450 HLp, Cytochrome P450 NF 25, Cytochrome P450 PCN1, HLP, NF 25, Nifedipine oxidase, P450C3, P450PCN1, Quinine 3 monooxygenase

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Validation Data Gallery

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  WB analysis of mouse liver using 67110-1-Ig (same clone as 67110-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 67110-1-Ig (CYP3A4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67110-1-Ig (same clone as 67110-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67110-1-Ig (CYP3A4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig liver using 67110-1-Ig (same clone as 67110-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 67110-1-Ig (CYP3A4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig gall bladder using 67110-1-Ig (same clone as 67110-1-PBS)

  pig gall bladder tissue were subjected to SDS PAGE followed by western blot with 67110-1-Ig (CYP3A4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse lung using 67110-1-Ig (same clone as 67110-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 67110-1-Ig (CYP3A4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 67110-1-Ig (same clone as 67110-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67110-1-Ig (CYP3A4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 67110-1-Ig (same clone as 67110-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67110-1-Ig (CYP3A4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67110-1-Ig (same clone as 67110-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67110-1-Ig (CYP3A4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67110-1-Ig (same clone as 67110-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67110-1-Ig (CYP3A4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse liver using 67110-1-Ig (same clone as 67110-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CYP3A4 antibody (67110-1-Ig, Clone: 2F11C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse liver using 67110-1-Ig (same clone as 67110-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CYP3A4 antibody (67110-1-Ig, Clone: 2F11C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat liver using 67110-1-Ig (same clone as 67110-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat liver tissue using CYP3A4 antibody (67110-1-Ig, Clone: 2F11C4 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 67110-1-Ig (same clone as 67110-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CYP3A4 antibody (67110-1-Ig, Clone: 2F11C4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67110-1-PBS targets CYP3A4 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CYP3A4 fusion protein Ag13074
• Full Name: cytochrome P450, family 3, subfamily A, polypeptide 4
• Calculated Molecular Weight: 503 aa, 57 kDa
• Observed Molecular Weight: 51-57 kDa
• GenBank Accession Number: BC101631
• Gene Symbol: CYP3A4
• Gene ID (NCBI): 1576
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.