CYP3A4 Monoclonal antibody, PBS Only (Detector)

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  WB analysis of mouse liver using 67110-1-Ig (same clone as 67110-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 67110-1-Ig (CYP3A4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

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  WB analysis using 67110-1-Ig (same clone as 67110-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67110-1-Ig (CYP3A4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

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  WB analysis of pig liver using 67110-1-Ig (same clone as 67110-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 67110-1-Ig (CYP3A4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

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  WB analysis of pig gall bladder using 67110-1-Ig (same clone as 67110-1-PBS)

  pig gall bladder tissue were subjected to SDS PAGE followed by western blot with 67110-1-Ig (CYP3A4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse lung using 67110-1-Ig (same clone as 67110-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 67110-1-Ig (CYP3A4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 67110-1-Ig (same clone as 67110-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67110-1-Ig (CYP3A4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 67110-1-Ig (same clone as 67110-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67110-1-Ig (CYP3A4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67110-1-Ig (same clone as 67110-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67110-1-Ig (CYP3A4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67110-1-Ig (same clone as 67110-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67110-1-Ig (CYP3A4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 67110-1-Ig (same clone as 67110-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CYP3A4 antibody (67110-1-Ig, Clone: 2F11C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 67110-1-Ig (same clone as 67110-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CYP3A4 antibody (67110-1-Ig, Clone: 2F11C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

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  IF Staining of rat liver using 67110-1-Ig (same clone as 67110-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat liver tissue using CYP3A4 antibody (67110-1-Ig, Clone: 2F11C4 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67110-1-Ig (same clone as 67110-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CYP3A4 antibody (67110-1-Ig, Clone: 2F11C4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67110-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50718-4

  Cytometric bead array standard curve of MP50718-4, CYP3A4 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67110-6-PBS. Detection antibody: 67110-1-PBS. Standard:Ag13074. Range: 0.781-100 ng/mL.

CYP3A4 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Cat No. 67110-1-PBS

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Synonyms

CYP3A3, CYP3A, CP34, CP33, Cholesterol 25-hydroxylase

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Product Information

67110-1-PBS targets CYP3A4 as part of a matched antibody pair:

MP50718-4: 67110-6-PBS capture and 67110-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CYP3A4 fusion protein Ag13074
• Full Name: cytochrome P450, family 3, subfamily A, polypeptide 4
• Calculated Molecular Weight: 503 aa, 57 kDa
• Observed Molecular Weight: 51-57 kDa
• GenBank Accession Number: BC101631
• Gene Symbol: CYP3A4
• Gene ID (NCBI): 1576
• RRID: AB_2882414
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P08684
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.