Filter: AllWBIHCIF-PIF/ICC

WB analysis of mouse liver using 67110-1-Ig
mouse liver tissue were subjected to SDS PAGE followed by western blot with 67110-1-Ig (CYP3A4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis using 67110-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67110-1-Ig (CYP3A4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of pig liver using 67110-1-Ig
pig liver tissue were subjected to SDS PAGE followed by western blot with 67110-1-Ig (CYP3A4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of pig gall bladder using 67110-1-Ig
pig gall bladder tissue were subjected to SDS PAGE followed by western blot with 67110-1-Ig (CYP3A4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 67110-1-Ig
mouse lung tissue were subjected to SDS PAGE followed by western blot with 67110-1-Ig (CYP3A4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IHC staining of mouse liver using 67110-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67110-1-Ig (CYP3A4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 67110-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67110-1-Ig (CYP3A4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 67110-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67110-1-Ig (CYP3A4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 67110-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67110-1-Ig (CYP3A4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse liver using 67110-1-Ig
Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CYP3A4 antibody (67110-1-Ig, Clone: 2F11C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
IF Staining of mouse liver using 67110-1-Ig
Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CYP3A4 antibody (67110-1-Ig, Clone: 2F11C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
IF Staining of rat liver using 67110-1-Ig
Immunofluorescent analysis of (4% PFA) fixed rat liver tissue using CYP3A4 antibody (67110-1-Ig, Clone: 2F11C4 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
IF Staining of HepG2 using 67110-1-Ig
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CYP3A4 antibody (67110-1-Ig, Clone: 2F11C4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

CYP3A4 Monoclonal antibody

Name: CYP3A4 Monoclonal antibody
Brand: Proteintech
SKU: 67110-1-Ig

CYP3A4 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Cat No. 67110-1-Ig

Clone No.2F11C4

Publications(4)

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Synonyms

2F11C4, Cholesterol 25-hydroxylase, CP33, CP34, CYP3A

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information
Publications (4)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	mouse liver tissue, pig liver tissue, pig gall bladder tissue, mouse lung tissue, rat liver tissue
Positive IHC detected in	mouse liver tissue, human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse liver tissue, rat liver tissue
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:300-1:1200
Immunofluorescence (IF)-P	IF-P : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below
IF	See 3 publications below

Product Information

67110-1-Ig targets CYP3A4 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity	human, mouse, rat, pig
Cited Reactivity	human, mouse
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag13074 Product name: Recombinant human CYP3A4 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 154-503 aa of BC101631 Sequence: DVLVRNLRREAETGKPVTLKDVFGAYSMDVITSTSFGVNIDSLNNPQDPFVENTKKLLRFDFLDPFFLSITVFPFLIPILEVLNICVFPREVTNFLRKSVKRMKESRLEDTQKHRVDFLQLMIDSQNSKETESHKALSDLELVAQSIIFIFAGYETTSSVLSFIMYELATHPDVQQKLQEEIDAVLPNKAPPTYDTVLQMEYLDMVVNETLRLFPIAMRLERVCKKDVEINGMFIPKGVVVMIPSYALHRDPKYWTEPEKFLPERFSKKNKDNIDPYIYTPFGSGPRNCIGMRFALMNMKLALIRVLQNFSFKPCKETQIPLKLSLGGLLQPEKPVVLKVESRDGTVSGA Predict reactive species
Full Name	cytochrome P450, family 3, subfamily A, polypeptide 4
Calculated Molecular Weight	503 aa, 57 kDa
Observed Molecular Weight	51-57 kDa
GenBank Accession Number	BC101631
Gene Symbol	CYP3A4
Gene ID (NCBI)	1576
RRID	AB_2882414
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P08684
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for CYP3A4 antibody 67110-1-Ig	Download protocol
IHC protocol for CYP3A4 antibody 67110-1-Ig	Download protocol
WB protocol for CYP3A4 antibody 67110-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Biomaterials Hepatic endoplasmic reticulum-derived nanodiscs for broad-spectrum drug detoxification Authors - Lei Sun View Article
human	IF	Biomed Rep Phenotypical, functional and transcriptomic comparison of two modified methods of hepatocyte differentiation from human induced pluripotent stem cells. Authors - Rong Li View Article
human	IF	Methods Mol Biol Homogeneous Differentiation of Functional Hepatocytes from Human Induced Pluripotent Stem Cells. Authors - Rong Li View Article
human	IF	Acta Biomater Hepar-on-a-sensor-platform with hybridization chain reaction amplification strategy to intuitively monitor the hepatoxicity of natural compounds Authors - Wenhui Qian View Article