WB result of CYP2W1 antibody (67419-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CYP2W1 transfected HEK-293 cells.
WB result of CYP2W1 antibody (67419-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CYP2W1 transfected HEK-293 cells.
WB analysis using 67419-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67419-1-Ig (CYP2W1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 67419-1-Ig (CYP2W1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC staining of human colon cancer using 67419-1-Ig
Immunohistochemical analysis of paraffin-embedded colon cancer slide using 67419-1-Ig (CYP2W1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded colon cancer slide using 67419-1-Ig (CYP2W1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 67419-1-Ig
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CYP2W1 antibody (67419-1-Ig, Clone: 2B10H2 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red).
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CYP2W1 antibody (67419-1-Ig, Clone: 2B10H2 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red).
FC experiment of HUVEC using 67419-1-Ig
1x10^6 HUVEC cells were intracellularly stained with 0.4 ug CYP2W1 Monoclonal antibody (67419-1-Ig, Clone:2B10H2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1x10^6 HUVEC cells were intracellularly stained with 0.4 ug CYP2W1 Monoclonal antibody (67419-1-Ig, Clone:2B10H2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IHC detected in
human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HepG2 cells
Positive FC (Intra) detected in
HUVEC cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:30000
Immunohistochemistry (IHC)
IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICC
IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)
FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
67419-1-Ig targets CYP2W1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
Cytochrome P450s have been proposed to be of importance both in carcinogenesis, by activating precarcinogens, and as determinants of cancer chemotherapy, where they participate in activation or inactivation of anti-cancer drugs. CYP2W1 is exclusively expressed in transformed tissue in the adult human, mainly in colon tumors. It is a potential drug target in cancer therapy. CYP2W1 has a molecular weight of 52-54 kDa, and 30/45 kDa of potential isoforms.
WB result of CYP2W1 antibody (67419-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CYP2W1 transfected HEK-293 cells.
WB analysis using 67419-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67419-1-Ig (CYP2W1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human colon cancer using 67419-1-Ig
Immunohistochemical analysis of paraffin-embedded colon cancer slide using 67419-1-Ig (CYP2W1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF/ICC Figures
IF Staining of HepG2 using 67419-1-Ig
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CYP2W1 antibody (67419-1-Ig, Clone: 2B10H2 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red).
FC (INTRA) Figures
FC experiment of HUVEC using 67419-1-Ig
1x10^6 HUVEC cells were intracellularly stained with 0.4 ug CYP2W1 Monoclonal antibody (67419-1-Ig, Clone:2B10H2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
CYP2W1 Monoclonal antibody
Catalog Number
67419-1-Ig
Citations
-
Dilutions
WB : 1:5000-1:30000 IHC : 1:1000-1:4000 IF/ICC : 1:200-1:800 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Applications
WB, IHC, IF/ICC, FC (Intra), ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
