CYP2W1 Monoclonal antibody, PBS Only

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• KD/KO Validated

CYP2W1 Monoclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B10H2

Cat no : 67419-1-PBS

Synonyms

EC:1.14.14.-, Cytochrome P450 2W1, CYPIIW1, 2B10H2

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Validation Data Gallery

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  WB analysis of HEK-293 using 67419-1-Ig (same clone as 67419-1-PBS)

  WB result of CYP2W1 antibody (67419-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CYP2W1 transfected HEK-293 cells. This data was developed using the same antibody clone with 67419-1-PBS in a different storage buffer formulation.

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  WB analysis using 67419-1-Ig (same clone as 67419-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67419-1-Ig (CYP2W1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67419-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67419-1-Ig (same clone as 67419-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CYP2W1 antibody (67419-1-Ig, Clone: 2B10H2 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67419-1-PBS in a different storage buffer formulation.

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  FC experiment of HUVEC using 67419-1-Ig (same clone as 67419-1-PBS)

  1x10^6 HUVEC cells were intracellularly stained with 0.4 ug CYP2W1 Monoclonal antibody (67419-1-Ig, Clone:2B10H2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67419-1-PBS in a different storage buffer formulation.

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Product Information

67419-1-PBS targets CYP2W1 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CYP2W1 fusion protein Ag29982
• Full Name: cytochrome P450, family 2, subfamily W, polypeptide 1
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 45-54 kDa
• GenBank Accession Number: BC025761
• Gene Symbol: CYP2W1
• Gene ID (NCBI): 54905
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Cytochrome P450s have been proposed to be of importance both in carcinogenesis, by activating precarcinogens, and as determinants of cancer chemotherapy, where they participate in activation or inactivation of anti-cancer drugs. CYP2W1 is exclusively expressed in transformed tissue in the adult human, mainly in colon tumors. It is a potential drug target in cancer therapy. CYP2W1 has a molecular weight of 52-54 kDa, and 30/45 kDa of potential isoforms.