CoraLite® Plus 488-conjugated CYP2W1 Monoclonal antibody

• Featured Product
• KD/KO Validated

CYP2W1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2B10H2

Cat no : CL488-67419

Synonyms

EC:1.14.14.-, Cytochrome P450 2W1, CYPIIW1, 2B10H2

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-67419

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 CYP2W1 antibody (CL488-67419, Clone: 2B10H2 ) at dilution of 1:200, CL594-Phalloidin (red).

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  FC experiment of HUVEC using CL488-67419

  1x10^6 HUVEC cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated CYP2W1 Monoclonal antibody (CL488-67419, Clone:2B10H2)(red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HUVEC cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67419 targets CYP2W1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CYP2W1 fusion protein Ag29982
• Full Name: cytochrome P450, family 2, subfamily W, polypeptide 1
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 45-54 kDa
• GenBank Accession Number: BC025761
• Gene Symbol: CYP2W1
• Gene ID (NCBI): 54905
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Cytochrome P450s have been proposed to be of importance both in carcinogenesis, by activating precarcinogens, and as determinants of cancer chemotherapy, where they participate in activation or inactivation of anti-cancer drugs. CYP2W1 is exclusively expressed in transformed tissue in the adult human, mainly in colon tumors. It is a potential drug target in cancer therapy. CYP2W1 has a molecular weight of 52-54 kDa, and 30/45 kDa of potential isoforms.