Anti-Human CSF2RA/CD116 Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using 98066-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CSF2RA/CD116 Rabbit Recombinant Antibody (98066-1-RR, Clone:241266B11) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L), or 0.25 ug Isotype Control. Cells were then stained with APC Anti-Human CD14 (26IC) (APC-65246, Clone: 26IC). Cells were not fixed.

• 

  FC experiment of human PBMCs using 98066-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CSF2RA/CD116 Rabbit Recombinant Antibody (98066-1-RR, Clone:241266B11) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (red), or 0.25 ug Isotype Control (blue). Cells were not fixed.

• 

  FC experiment of human PBMCs using 98066-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CSF2RA/CD116 Rabbit Recombinant Antibody (98066-1-RR, Clone:241266B11) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L), and 0.25 ug APC Anti-Human CD14 (26IC) (APC-65246, Clone: 26IC), and 0.25 ug Isotype Control. Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98066-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98066-1-RR against Human CSF2RA/CD116 were performed. The affinity constant is 1.52 nM.

CSF2RA/CD116 {ptg:Brand} Recombinant Antibody for FC

Cat No. 98066-1-RR

Clone No.241266B11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CD116, 241266B11, GMCSFR, GM CSF R alpha, CSF2RA

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98066-1-RR targets CSF2RA/CD116 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage)
• Calculated Molecular Weight: 46 kDa
• GenBank Accession Number: BC002635
• Gene Symbol: CD116
• Gene ID (NCBI): 1438
• RRID: AB_3672213
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P15509
• Storage Buffer: PBS with 0.09% sodium azide , pH 7.3
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CD116, also named as CSF2RA CDw116 CSF2RAX CSF2RAY CSF2RX CSF2RY GM-CSF-R-alpha GMCSFR and GMR, is a low affinity receptor for granulocyte-macrophage colony-stimulating factor. CD116 transduces a signal that results in the proliferation, differentiation, and functional activation of hematopoietic cells.