CoraLite® Plus 405 Anti-Human CSF2RA/CD116 Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using CL405-98066

  1x10^6 human PBMCs were surface stained with APC Anti-Human CD14 and 0.25 ug CoraLite® Plus 405 Anti-Human CSF2RA/CD116 Rabbit Recombinant Antibody (CL405-98066, Clone:241266B11), or CoraLite® Plus 405 Rabbit IgG Isotype Control Recombinant Antibody (CL405-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using CL405-98066

  1x10^6 human PBMCs were surface stained with 0.25 ug CoraLite® Plus 405 Anti-Human CSF2RA/CD116 Rabbit Recombinant Antibody (CL405-98066, Clone: 241266B11) (red), or CoraLite® Plus 405 Rabbit IgG Isotype Control Recombinant Antibody (CL405-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

CSF2RA/CD116 Recombinant Antibody for FC

Cat No. CL405-98066

Clone No.241266B11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CD116, 241266B11, GMCSFR, GM CSF R alpha, CSF2RA

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL405-98066 targets CSF2RA/CD116 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage)
• Calculated Molecular Weight: 46 kDa
• GenBank Accession Number: BC002635
• Gene Symbol: CD116
• Gene ID (NCBI): 1438
• RRID: AB_3672423
• Conjugate: CoraLite® Plus 405 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 399 nm / 422 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P15509
• Storage Buffer: PBS with 0.09% sodium azide , pH 7.3
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD116, also named as CSF2RA CDw116 CSF2RAX CSF2RAY CSF2RX CSF2RY GM-CSF-R-alpha GMCSFR and GMR, is a low affinity receptor for granulocyte-macrophage colony-stimulating factor. CD116 transduces a signal that results in the proliferation, differentiation, and functional activation of hematopoietic cells.