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APC Anti-Human CD14 (26IC)

CD14 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

FC

Conjugate

APC Fluorescent Dye

CloneNo.

26IC

Cat no : APC-65246

Synonyms

CD14, CD14 molecule

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using APC Anti-Human CD14 (26IC) (APC-65246)

    FC experiment of human PBMCs using APC-65246

    1X10^6 human PBMCs were surface stained with 5 ul APC Anti-Human CD14 (APC-65246, Clone:26IC) (red) or unstained. Cells were not fixed. Monocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using APC Anti-Human CD14 (26IC) (APC-65246)

    FC experiment of human PBMCs using APC-65246

    1X10^6 human PBMCs were surface stained with 5 ul APC Anti-Human CD14 (APC-65246, Clone:26IC) or unstained. Cells were not fixed. Monocytes were gated.

Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

APC-65246 targets CD14 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen N/A
Full Name CD14 molecule
Calculated Molecular Weight 375 aa, 40 kDa
GenBank Accession NumberBC010507
Gene Symbol CD14
Gene ID (NCBI) 929
Conjugate APC Fluorescent Dye
Excitation/Emission Maxima Wavelengths650 nm / 660 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.

Protocols

Product Specific Protocols
FC protocol for APC CD14 antibody APC-65246Download protocol
Standard Protocols
Click here to view our Standard Protocols