CRTC2,TORC2 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

CRTC2,TORC2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12497-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 12497-1-AP (same clone as 12497-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12497-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 12497-1-AP (same clone as 12497-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12497-1-AP (CRTC2,TORC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12497-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 12497-1-AP (same clone as 12497-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12497-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 12497-1-AP (same clone as 12497-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12497-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 12497-1-AP (same clone as 12497-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas using 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 12497-1-PBS in a different storage buffer formulation.

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  IHC staining of rat kidney using 12497-1-AP (same clone as 12497-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12497-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 12497-1-AP (same clone as 12497-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12497-1-PBS in a different storage buffer formulation.

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  IHC staining of human urothelial carcinoma using 12497-1-AP (same clone as 12497-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12497-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 12497-1-AP (same clone as 12497-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12497-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 12497-1-AP (same clone as 12497-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12497-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 12497-1-AP (same clone as 12497-1-PBS)

  Immunofluorescent analysis of Hela cells, using CRTC2 antibody 12497-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 12497-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 12497-1-AP (same clone as 12497-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12497-1-PBS in a different storage buffer formulation.

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Product Information

12497-1-PBS targets CRTC2,TORC2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CRTC2,TORC2 fusion protein Ag3167
• Full Name: CREB regulated transcription coactivator 2
• Calculated Molecular Weight: 693 aa, 73 kDa
• Observed Molecular Weight: 73 kDa
• GenBank Accession Number: BC053562
• Gene Symbol: TORC2
• Gene ID (NCBI): 200186
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CRTC2, also named as TORC2, belongs to the TORC family. It is a transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. It acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. CRTC2 enhances the interaction of CREB1 with TAF4. Ir regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. CRTC2 regulates the expression of specific genes such as the steroidogenic gene, StAR. TORC2 was recently shown to be an important regulator of gluconeogenesis in the livers of mammals. It is one of the other key regulators of CRE-dependent MIE gene expression in NT2 cells. This regulation is linked to VIP-induced TORC2 dephosphorylation and translocation to the nucleus. (PMID: 19369332, 20504934 ) .