CRTC2,TORC2 Polyclonal antibody

• Featured Product
• KD/KO Validated

CRTC2,TORC2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

23

Cat no : 12497-1-AP

Synonyms

CRTC2, CREB-regulated transcription coactivator 2, Transducer of regulated cAMP response element-binding protein 2, Transducer of CREB protein 2, TORC2

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Validation Data Gallery

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  WB analysis using 12497-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 12497-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12497-1-AP (CRTC2,TORC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 12497-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12497-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 12497-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of rat kidney using 12497-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 12497-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 12497-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 12497-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 12497-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 12497-1-AP

  Immunofluorescent analysis of Hela cells, using CRTC2 antibody 12497-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  IF Staining of HeLa using 12497-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, mouse liver tissue, HEK-293 cells, mouse kidney, rat kidney
• Positive IHC detected in: human skin cancer tissue, human gliomas tissue, rat kidney tissue, human urothelial carcinoma tissue, mouse lung tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 18 publications below
• IHC: See 5 publications below
• IF: See 7 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

12497-1-AP targets CRTC2,TORC2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CRTC2,TORC2 fusion protein Ag3167
• Full Name: CREB regulated transcription coactivator 2
• Calculated Molecular Weight: 693 aa, 73 kDa
• Observed Molecular Weight: 73 kDa
• GenBank Accession Number: BC053562
• Gene Symbol: TORC2
• Gene ID (NCBI): 200186
• RRID: AB_2260910
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CRTC2, also named as TORC2, belongs to the TORC family. It is a transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. It acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. CRTC2 enhances the interaction of CREB1 with TAF4. Ir regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. CRTC2 regulates the expression of specific genes such as the steroidogenic gene, StAR. TORC2 was recently shown to be an important regulator of gluconeogenesis in the livers of mammals. It is one of the other key regulators of CRE-dependent MIE gene expression in NT2 cells. This regulation is linked to VIP-induced TORC2 dephosphorylation and translocation to the nucleus. (PMID: 19369332, 20504934 ) .

Publications

Species	Application	Title
human,mouse	WB,IHC,IF,IP	Adv Sci (Weinh); cAMP-Induced Nuclear Condensation of CRTC2 Promotes Transcription Elongation and Cystogenesis in Autosomal Dominant Polycystic Kidney Disease.; Authors - Zeyun Mi ; KD Validated
mouse	WB	Mol Cell; LKB1 controls inflammatory potential through CRTC2-dependent histone acetylation; Authors - Shelby E Compton
human	IHC	Am J Transplant; The tacrolimus-induced glucose homeostasis imbalance in terms of the liver: From bench to bedside.; Authors - Qi Ling
mouse	IHC,IF	J Neurosci; Clock and light regulation of the CREB coactivator CRTC1 in the suprachiasmatic circadian clock.; Authors - Sakamoto Kensuke K
mouse	WB	Biochem Pharmacol; Piperlongumine, a natural alkaloid from Piper longum L. ameliorates metabolic-associated fatty liver disease by antagonizing the thromboxane A2 receptor; Authors - Yufeng Dai
human,mouse,bovine	WB,IF	J Neurosci; CREB Coactivator CRTC2 Plays a Crucial Role in Endothelial Function.; Authors - Hideaki Kanki

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