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  • Featured Product
  • KD/KO Validated

CRTC2,TORC2 Polyclonal antibody

CRTC2,TORC2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 12497-1-AP

Synonyms

CRTC2, CREB-regulated transcription coactivator 2, Transducer of regulated cAMP response element-binding protein 2, Transducer of CREB protein 2, TORC2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CRTC2,TORC2 Polyclonal antibody (12497-1-AP)

    WB analysis using 12497-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using CRTC2,TORC2 Polyclonal antibody (12497-1-AP)

    WB analysis of mouse liver using 12497-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using CRTC2,TORC2 Polyclonal antibody (12497-1-AP)

    WB analysis of HEK-293 using 12497-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using CRTC2,TORC2 Polyclonal antibody (12497-1-AP)

    WB analysis of mouse liver using 12497-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 12497-1-AP (CRTC2,TORC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using CRTC2,TORC2 Polyclonal antibody (12497-1-AP)

    IHC staining of human gliomas using 12497-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using CRTC2,TORC2 Polyclonal antibody (12497-1-AP)

    IHC staining of rat kidney using 12497-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using CRTC2,TORC2 Polyclonal antibody (12497-1-AP)

    IHC staining of human skin cancer using 12497-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using CRTC2,TORC2 Polyclonal antibody (12497-1-AP)

    IHC staining of human urothelial carcinoma using 12497-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using CRTC2,TORC2 Polyclonal antibody (12497-1-AP)

    IHC staining of mouse lung using 12497-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using CRTC2,TORC2 Polyclonal antibody (12497-1-AP)

    IHC staining of mouse kidney using 12497-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CRTC2,TORC2 Polyclonal antibody (12497-1-AP)

    IF Staining of HeLa using 12497-1-AP

    Immunofluorescent analysis of Hela cells, using CRTC2 antibody 12497-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CRTC2,TORC2 Polyclonal antibody (12497-1-AP)

    IF Staining of HeLa using 12497-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse kidney tissue, mouse liver tissue, HEK-293 cells, mouse kidney, rat kidney
Positive IHC detected inhuman skin cancer tissue, human gliomas tissue, rat kidney tissue, human urothelial carcinoma tissue, mouse lung tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12497-1-AP targets CRTC2,TORC2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CRTC2,TORC2 fusion protein Ag3167
Full Name CREB regulated transcription coactivator 2
Calculated Molecular Weight 693 aa, 73 kDa
Observed Molecular Weight 73 kDa
GenBank Accession NumberBC053562
Gene Symbol TORC2
Gene ID (NCBI) 200186
RRIDAB_2260910
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CRTC2, also named as TORC2, belongs to the TORC family. It is a transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. It acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. CRTC2 enhances the interaction of CREB1 with TAF4. Ir regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. CRTC2 regulates the expression of specific genes such as the steroidogenic gene, StAR. TORC2 was recently shown to be an important regulator of gluconeogenesis in the livers of mammals. It is one of the other key regulators of CRE-dependent MIE gene expression in NT2 cells. This regulation is linked to VIP-induced TORC2 dephosphorylation and translocation to the nucleus. (PMID: 19369332, 20504934 ) .

Protocols

Product Specific Protocols
WB protocol for CRTC2,TORC2 antibody 12497-1-APDownload protocol
IHC protocol for CRTC2,TORC2 antibody 12497-1-APDownload protocol
IF protocol for CRTC2,TORC2 antibody 12497-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC,IF,IP

Adv Sci (Weinh)

cAMP-Induced Nuclear Condensation of CRTC2 Promotes Transcription Elongation and Cystogenesis in Autosomal Dominant Polycystic Kidney Disease.

Authors - Zeyun Mi
  • KD Validated
View Article
mouseWB

Mol Cell

LKB1 controls inflammatory potential through CRTC2-dependent histone acetylation

Authors - Shelby E Compton
View Article
humanIHC

Am J Transplant

The tacrolimus-induced glucose homeostasis imbalance in terms of the liver: From bench to bedside.

Authors - Qi Ling
View Article
mouseIHC,IF

J Neurosci

Clock and light regulation of the CREB coactivator CRTC1 in the suprachiasmatic circadian clock.

Authors - Sakamoto Kensuke K
View Article
human,mouse,bovineWB,IF

J Neurosci

CREB Coactivator CRTC2 Plays a Crucial Role in Endothelial Function.

Authors - Hideaki Kanki
View Article
humanWB,IF

J Virol

Breaking human cytomegalovirus major immediate-early gene silence by vasoactive intestinal peptide stimulation of the protein kinase A-CREB-TORC2 signaling cascade in human pluripotent embryonal NTera2 cells.

Authors - Yuan Jinxiang J
View Article