CoraLite®594-conjugated CRABP2 Polyclonal antibody

Name: CoraLite®594-conjugated CRABP2 Polyclonal antibody, 100ul
Brand: Proteintech
SKU: CL594-10225-100UL

CRABP2 Polyclonal Antibody for IF/ICC, IF-P, FC (Intra)

Cat No. CL594-10225

Publications(1)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IF-P, FC (Intra)

Synonyms

CRABP-II, CRABP II, Cellular retinoic acid-binding protein II, Cellular retinoic acid-binding protein 2, Cellular retinoic acid binding protein II

Formulation: PBS, Proclin300, BSA, Glycerol

PBS, Proclin300, BSA, Glycerol

Conjugate: CoraLite®594

Unconjugated CoraLite®594

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Filter: AllIF-PIF/ICCFC (Intra)

IF Staining of human skin cancer using CL594-10225
Immunofluorescent analysis of (4% PFA) fixed human skin cancer tissue using CoraLite®594-conjugated CRABP2 antibody (CL594-10225) at dilution of 1:50.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of MCF-7 using CL594-10225
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite®594 CRABP2 antibody (CL594-10225) at dilution of 1:200.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of MCF-7 using CL594-10225
1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human CRABP2 (CL594-10225) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive IF-P detected in	human skin cancer tissue
Positive IF/ICC detected in	MCF-7 cells
Positive FC (Intra) detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-10225 targets CRABP2 in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag0309 Product name: Recombinant human CRABP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-138 aa of BC001109 Sequence: MPNFSGNWKIIRSENFEELLKVLGVNVMLRKIAVAAASKPAVEIKQEGDTFYIKTSTTVRTTEINFKVGEEFEEQTVDGRPCKSLVKWESENKMVCEQKLLKGEGPKTSWTRELTNDGELILTMTADDVVCTRVYVRE Predict reactive species
Full Name	cellular retinoic acid binding protein 2
Calculated Molecular Weight	16 kDa
GenBank Accession Number	BC001109
Gene Symbol	CRABP2
Gene ID (NCBI)	1382
RRID	AB_2883404
Conjugate	CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths	588 nm / 604 nm
Excitation Laser	Yellow-Green Laser (561 nm)
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P29373
Storage Buffer	PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage Conditions	Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Cellular retinoic acid binding protein 2 (CRABP2, synonyms: RBP6, CRABP-II). A number of specific carrier proteins for members of the vitamin A family have been discovered. Cellular retinoic acid binding proteins (CRABP) are low molecular weight proteins whose precise function remains unknown. CRABP2 is important in retinoic acid-mediated regulation of human skin growth and differentiation. It has been postulated that the CRABP2 gene is transcriptionally regulated by a newly synthesized regulatory protein.