CRABP2 Polyclonal antibody

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  WB analysis of HeLa using 10225-1-AP

  WB result of CRABP2 antibody (10225-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-CRABP2 transfected HeLa cells.

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  WB analysis using 10225-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10225-1-AP (CRABP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10225-1-AP

  IP result of anti-CRABP2 (IP:10225-1-AP, 3ug; Detection:10225-1-AP 1:300) with HeLa cells lysate 4650ug.

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  IHC staining of human skin using 10225-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 10225-1-AP (CRABP2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 10225-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 10225-1-AP (CRABP2 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of human skin cancer using 10225-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human skin cancer tissue using CRABP2 antibody (10225-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MCF-7 using 10225-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CRABP2 antibody (10225-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  FC experiment of MCF-7 using 10225-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human CRABP2 (10225-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

CRABP2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Cat No. 10225-1-AP

Publications(45)

Reviews (3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA

Synonyms

CRABP-II, CRABP II, Cellular retinoic acid-binding protein II, Cellular retinoic acid-binding protein 2, Cellular retinoic acid binding protein II

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HT-29 cells, MCF-7 cells, mouse embryo tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human skin cancer tissue
• Positive IF/ICC detected in: MCF-7 cells
• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 7 publications below
• WB: See 29 publications below
• IHC: See 15 publications below
• IF: See 15 publications below
• IP: See 3 publications below
• CoIP: See 2 publications below

Product Information

10225-1-AP targets CRABP2 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CRABP2 fusion protein Ag0309
• Full Name: cellular retinoic acid binding protein 2
• Calculated Molecular Weight: 16 kDa
• Observed Molecular Weight: 16 kDa
• GenBank Accession Number: BC001109
• Gene Symbol: CRABP2
• Gene ID (NCBI): 1382
• RRID: AB_2085455
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P29373
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cellular retinoic acid binding protein 2 (CRABP2, synonyms: RBP6, CRABP-II). A number of specific carrier proteins for members of the vitamin A family have been discovered. Cellular retinoic acid binding proteins (CRABP) are low molecular weight proteins whose precise function remains unknown. CRABP2 is important in retinoic acid-mediated regulation of human skin growth and differentiation. It has been postulated that the CRABP2 gene is transcriptionally regulated by a newly synthesized regulatory protein.

Publications

Species	Application	Title
mouse	IF	Science; A single progenitor population switches behavior to maintain and repair esophageal epithelium.; Authors - David P Doupé
human	WB	Mol Cell; TRIP12 promotes small-molecule-induced degradation through K29/K48-branched ubiquitin chains.; Authors - Ai Kaiho-Soma
human	WB,IF	Neuron; Divergent Hox Coding and Evasion of Retinoid Signaling Specifies Motor Neurons Innervating Digit Muscles.; Authors - Alana I Mendelsohn
mouse	IF	Nat Commun; The developing mouse coronal suture at single-cell resolution.; Authors - D'Juan T Farmer
mouse	IF	Dev Cell; Single-Cell Transcriptomic Analyses of the Developing Meninges Reveal Meningeal Fibroblast Diversity and Function.; Authors - John DeSisto
human	IF	Stem Cell Reports; BLIMP1 Is Required for Postnatal Epidermal Homeostasis but Does Not Define a Sebaceous Gland Progenitor under Steady-State Conditions.; Authors - Kai Kretzschmar

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Yan (Verified Customer) (01-27-2020)

I used this antibody on mouse embryonic section for the immunofluorescence. It worked very well.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Mouse embryonic section

FH

lunfeng (Verified Customer) (01-27-2020)

VERY GOOD

• Applications: Immunofluorescence,

FH

Jie (Verified Customer) (01-27-2020)

VERY GOOD ANTIBODY. WORKS IN KO VERSUS WT CONTROL MICE.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: mouse brain tissue