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CRABP2 Polyclonal antibody
CRABP2 Polyclonal Antibody for FC (Intra), IF, IHC, IP, WB, ELISA
Cat no : 10225-1-AP
Validation Data Gallery
|Positive WB detected in||HEK-293 cells, HeLa cells, HT-29 cells, MCF-7 cells, mouse embryo tissue|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human skin tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human skin cancer tissue|
|Positive FC detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
10225-1-AP targets CRABP2 in WB, IP, IHC, IF, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CRABP2 fusion protein Ag0309|
|Full Name||cellular retinoic acid binding protein 2|
|Calculated molecular weight||16 kDa|
|Observed molecular weight||16 kDa|
|GenBank accession number||BC001109|
|Gene ID (NCBI)||1382|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Cellular retinoic acid binding protein 2 (CRABP2, synonyms: RBP6, CRABP-II). A number of specific carrier proteins for members of the vitamin A family have been discovered. Cellular retinoic acid binding proteins (CRABP) are low molecular weight proteins whose precise function remains unknown. CRABP2 is important in retinoic acid-mediated regulation of human skin growth and differentiation. It has been postulated that the CRABP2 gene is transcriptionally regulated by a newly synthesized regulatory protein.
|Product Specific Protocols|
|WB protocol for CRABP2 antibody 10225-1-AP||Download protocol|
|IHC protocol for CRABP2 antibody 10225-1-AP||Download protocol|
|IF protocol for CRABP2 antibody 10225-1-AP||Download protocol|
|IP protocol for CRABP2 antibody 10225-1-AP||Download protocol|
|FC protocol for CRABP2 antibody 10225-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Yan (Verified Customer) (01-27-2020)
I used this antibody on mouse embryonic section for the immunofluorescence. It worked very well.
lunfeng (Verified Customer) (01-27-2020)
Jie (Verified Customer) (01-27-2020)
VERY GOOD ANTIBODY. WORKS IN KO VERSUS WT CONTROL MICE.