CRABP2 Polyclonal antibody

• Featured Product
• KD/KO Validated

CRABP2 Polyclonal Antibody for FC (Intra), IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC (Intra), CoIP, ELISA

Conjugate

Unconjugated

Publications

34

Reviews (3)

Cat no : 10225-1-AP

Synonyms

CRABP II, CRABP2, RBP6

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Validation Data Gallery

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  WB analysis of HeLa using 10225-1-AP

  WB result of CRABP2 antibody (10225-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-CRABP2 transfected HeLa cells.

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  WB analysis using 10225-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10225-1-AP (CRABP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10225-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10225-1-AP (CRABP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10225-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10225-1-AP (CRABP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10225-1-AP

  IP Result of anti-CRABP2 (IP:10225-1-AP, 3ug; Detection:10225-1-AP 1:300) with HeLa cells lysate 4650ug.

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  IHC staining of human skin using 10225-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 10225-1-AP (CRABP2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 10225-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 10225-1-AP (CRABP2 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of human skin cancer using 10225-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human skin cancer tissue using CRABP2 antibody (10225-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of MCF-7 using 10225-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human CRABP2 (10225-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HT-29 cells, MCF-7 cells, mouse embryo tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: human skin cancer tissue
• Positive FC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 6 publications below
• WB: See 22 publications below
• IHC: See 11 publications below
• IF: See 11 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below

Product Information

10225-1-AP targets CRABP2 in WB, IP, IHC, IF, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CRABP2 fusion protein Ag0309
• Full Name: cellular retinoic acid binding protein 2
• Calculated molecular weight: 16 kDa
• Observed molecular weight: 16 kDa
• GenBank accession number: BC001109
• Gene symbol: CRABP2
• Gene ID (NCBI): 1382
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cellular retinoic acid binding protein 2 (CRABP2, synonyms: RBP6, CRABP-II). A number of specific carrier proteins for members of the vitamin A family have been discovered. Cellular retinoic acid binding proteins (CRABP) are low molecular weight proteins whose precise function remains unknown. CRABP2 is important in retinoic acid-mediated regulation of human skin growth and differentiation. It has been postulated that the CRABP2 gene is transcriptionally regulated by a newly synthesized regulatory protein.

Publications

Species	Application	Title
mouse	IF	Science; A single progenitor population switches behavior to maintain and repair esophageal epithelium.; Authors - David P Doupé
human	WB	Mol Cell; TRIP12 promotes small-molecule-induced degradation through K29/K48-branched ubiquitin chains.; Authors - Ai Kaiho-Soma
human	WB,IF	Neuron; Divergent Hox Coding and Evasion of Retinoid Signaling Specifies Motor Neurons Innervating Digit Muscles.; Authors - Alana I Mendelsohn
mouse	IF	Nat Commun; The developing mouse coronal suture at single-cell resolution.; Authors - D'Juan T Farmer
mouse	IF	Dev Cell; Single-Cell Transcriptomic Analyses of the Developing Meninges Reveal Meningeal Fibroblast Diversity and Function.; Authors - John DeSisto
human	IF	Stem Cell Reports; BLIMP1 Is Required for Postnatal Epidermal Homeostasis but Does Not Define a Sebaceous Gland Progenitor under Steady-State Conditions.; Authors - Kai Kretzschmar

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Yan (Verified Customer) (01-27-2020)

I used this antibody on mouse embryonic section for the immunofluorescence. It worked very well.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Mouse embryonic section

FH

lunfeng (Verified Customer) (01-27-2020)

VERY GOOD

• Applications: Immunofluorescence,

FH

Jie (Verified Customer) (01-27-2020)

VERY GOOD ANTIBODY. WORKS IN KO VERSUS WT CONTROL MICE.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: mouse brain tissue

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