- Featured Product
- KD/KO Validated
CRABP2 Polyclonal antibody
CRABP2 Polyclonal Antibody for FC (Intra), IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, FC (Intra), CoIP, ELISA
Conjugate
Unconjugated
Cat no : 10225-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, HeLa cells, HT-29 cells, MCF-7 cells, mouse embryo tissue |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | human skin cancer tissue |
Positive FC detected in | MCF-7 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 6 publications below |
WB | See 22 publications below |
IHC | See 11 publications below |
IF | See 11 publications below |
IP | See 2 publications below |
CoIP | See 2 publications below |
Product Information
10225-1-AP targets CRABP2 in WB, IP, IHC, IF, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CRABP2 fusion protein Ag0309 |
Full Name | cellular retinoic acid binding protein 2 |
Calculated molecular weight | 16 kDa |
Observed molecular weight | 16 kDa |
GenBank accession number | BC001109 |
Gene symbol | CRABP2 |
Gene ID (NCBI) | 1382 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Cellular retinoic acid binding protein 2 (CRABP2, synonyms: RBP6, CRABP-II). A number of specific carrier proteins for members of the vitamin A family have been discovered. Cellular retinoic acid binding proteins (CRABP) are low molecular weight proteins whose precise function remains unknown. CRABP2 is important in retinoic acid-mediated regulation of human skin growth and differentiation. It has been postulated that the CRABP2 gene is transcriptionally regulated by a newly synthesized regulatory protein.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for CRABP2 antibody 10225-1-AP | Download protocol |
IHC protocol for CRABP2 antibody 10225-1-AP | Download protocol |
IF protocol for CRABP2 antibody 10225-1-AP | Download protocol |
IP protocol for CRABP2 antibody 10225-1-AP | Download protocol |
FC protocol for CRABP2 antibody 10225-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Science A single progenitor population switches behavior to maintain and repair esophageal epithelium. | ||
Mol Cell TRIP12 promotes small-molecule-induced degradation through K29/K48-branched ubiquitin chains. | ||
Neuron Divergent Hox Coding and Evasion of Retinoid Signaling Specifies Motor Neurons Innervating Digit Muscles. | ||
Dev Cell Single-Cell Transcriptomic Analyses of the Developing Meninges Reveal Meningeal Fibroblast Diversity and Function. | ||
Stem Cell Reports BLIMP1 Is Required for Postnatal Epidermal Homeostasis but Does Not Define a Sebaceous Gland Progenitor under Steady-State Conditions. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Yan (Verified Customer) (01-27-2020) | I used this antibody on mouse embryonic section for the immunofluorescence. It worked very well.
|
FH lunfeng (Verified Customer) (01-27-2020) | VERY GOOD
|
FH Jie (Verified Customer) (01-27-2020) | VERY GOOD ANTIBODY. WORKS IN KO VERSUS WT CONTROL MICE.
|