CoraLite®594-conjugated CPLX2 Monoclonal antibody
CPLX2 Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat
Applications
FC (Intra)
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
1B2A11
Cat no : CL594-66849
Synonyms
Validation Data Gallery
Tested Applications
Positive FC (Intra) detected in | SH-SY5Y cells |
Positive FC detected in | SH-SY5Y cells |
Recommended dilution
Application | Dilution |
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Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL594-66849 targets CPLX2 in FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.
Tested Reactivity | Human, Mouse, Rat |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | CPLX2 fusion protein Ag27949 |
Full Name | complexin 2 |
Calculated Molecular Weight | 134 aa, 15 kDa |
Observed Molecular Weight | 18-20 kDa |
GenBank Accession Number | BC093706 |
Gene Symbol | CPLX2 |
Gene ID (NCBI) | 10814 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Complexins are soluble proteins that regulate the activity of soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complexes necessary for vesicle fusion. Neuronal specific complexin 1 (CPLX1) has inhibitory and stimulatory effects on exocytosis by clamping trans-SNARE complexes in a prefusion state and promoting conformational changes to facilitate membrane fusion following cell stimulation. Complexin2 (CPLX2) is a pre-synaptic protein believed to regulate neurotransmitter release from pre-synaptic terminals, it is downregulated in schizophrenic patients suffering from depression, animal models of depression and neurological disorders such as Huntington's disease in which depression is a major symptom.
Protocols
Product Specific Protocols | |
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FC protocol for CL594 CPLX2 antibody CL594-66849 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |