CPLX2 Monoclonal antibody

CPLX2 Monoclonal Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 66849-1-Ig
Clone No.1B2A11

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, FC (Intra), ELISA

CPX 2, CPLX 2, Complexin-2, 921L, 921 L

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite®594
Size/Concentration:  150 μL, 1900 ug/ml
20 μL, 1900 ug/ml
150 μL, 1900 ug/ml

$449/ 150 μL

Freight/Packing: $40.00

Quantity

Buy two full size antibodies and get a third full size primary antibody for free. Use promo code B2G125.


Proteintech Guarantee



Tested Applications

Positive WB detected inmouse brain tissue, mouse cerebellum tissue, rat brain tissue, rat cerebellum tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66849-1-Ig targets CPLX2 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CPLX2 fusion protein Ag27949 Predict reactive species
Full Name complexin 2
Calculated Molecular Weight 134 aa, 15 kDa
Observed Molecular Weight 18-20 kDa
GenBank Accession NumberBC093706
Gene Symbol CPLX2
Gene ID (NCBI) 10814
RRIDAB_2882189
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ6PUV4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Complexins are soluble proteins that regulate the activity of soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complexes necessary for vesicle fusion. Neuronal specific complexin 1 (CPLX1) has inhibitory and stimulatory effects on exocytosis by clamping trans-SNARE complexes in a prefusion state and promoting conformational changes to facilitate membrane fusion following cell stimulation. Complexin2 (CPLX2) is a pre-synaptic protein believed to regulate neurotransmitter release from pre-synaptic terminals, it is downregulated in schizophrenic patients suffering from depression, animal models of depression and neurological disorders such as Huntington's disease in which depression is a major symptom.

Protocols

Product Specific Protocols
WB protocol for CPLX2 antibody 66849-1-IgDownload protocol
IHC protocol for CPLX2 antibody 66849-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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