COTL1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

COTL1 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4A3D9

Cat no : 60237-1-PBS

Synonyms

CLP, Coactosin like protein, COTL1

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Validation Data Gallery

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  WB analysis of A431 using 60237-1-Ig (same clone as 60237-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 60237-1-Ig (COTL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60237-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 60237-1-Ig (same clone as 60237-1-PBS)

  WB result of COTL1 antibody (60237-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-COTL1 transfected HeLa cells. This data was developed using the same antibody clone with 60237-1-PBS in a different storage buffer formulation.

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  WB analysis of human peripheral blood platelets using 60237-1-Ig (same clone as 60237-1-PBS)

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 60237-1-Ig (COTL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60237-1-PBS in a different storage buffer formulation.

• 

  WB analysis of fetal human brain using 60237-1-Ig (same clone as 60237-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60237-1-Ig (COTL1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60237-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 60237-1-Ig (same clone as 60237-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60237-1-Ig (COTL1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60237-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 60237-1-Ig (same clone as 60237-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60237-1-Ig (COTL1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60237-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 60237-1-Ig (same clone as 60237-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using COTL1 antibody (60237-1-Ig, Clone: 4A3D9 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60237-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60237-1-PBS targets COTL1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: COTL1 fusion protein Ag1230
• Full Name: coactosin-like 1 (Dictyostelium)
• Calculated Molecular Weight: 16 kDa
• Observed Molecular Weight: 14-16 kDa
• GenBank Accession Number: BC010884
• Gene Symbol: COTL1
• Gene ID (NCBI): 23406
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

COTL1, also named as CLP, binds to F-actin in a calcium-independent manner. And it has no direct effect on actin depolymerization.