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COTL1 Monoclonal antibody

COTL1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 60237-1-Ig
Clone No.4A3D9

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

CLP, Coactosin like protein, COTL1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inA431 cells, fetal human brain tissue, human peripheral blood platelets, HeLa cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60237-1-Ig targets COTL1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen COTL1 fusion protein Ag1230 Predict reactive species
Full Name coactosin-like 1 (Dictyostelium)
Calculated Molecular Weight 16 kDa
Observed Molecular Weight 14-16 kDa
GenBank Accession NumberBC010884
Gene Symbol COTL1
Gene ID (NCBI) 23406
RRIDAB_2881360
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ14019
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COTL1, also named as CLP, binds to F-actin in a calcium-independent manner. And it has no direct effect on actin depolymerization.

Protocols

Product Specific Protocols
WB protocol for COTL1 antibody 60237-1-IgDownload protocol
IHC protocol for COTL1 antibody 60237-1-IgDownload protocol
IF protocol for COTL1 antibody 60237-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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