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COTL1 Monoclonal antibody

COTL1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

4A3D9

Cat no : 60237-1-Ig

Synonyms

CLP, Coactosin like protein, COTL1

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using COTL1 Monoclonal antibody (60237-1-Ig)

    WB analysis of A431 using 60237-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 60237-1-Ig (COTL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using COTL1 Monoclonal antibody (60237-1-Ig)

    WB analysis of HeLa using 60237-1-Ig

    WB result of COTL1 antibody (60237-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-COTL1 transfected HeLa cells.

  • Western Blot (WB) analysis of human peripheral blood platelets using COTL1 Monoclonal antibody (60237-1-Ig)

    WB analysis of human peripheral blood platelets using 60237-1-Ig

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 60237-1-Ig (COTL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of fetal human brain tissue using COTL1 Monoclonal antibody (60237-1-Ig)

    WB analysis of fetal human brain using 60237-1-Ig

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60237-1-Ig (COTL1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using COTL1 Monoclonal antibody (60237-1-Ig)

    IHC staining of human lung cancer using 60237-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60237-1-Ig (COTL1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using COTL1 Monoclonal antibody (60237-1-Ig)

    IHC staining of human lung cancer using 60237-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60237-1-Ig (COTL1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using COTL1 Monoclonal antibody (60237-1-Ig)

    IF Staining of HepG2 using 60237-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using COTL1 antibody (60237-1-Ig, Clone: 4A3D9 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inA431 cells, fetal human brain tissue, human peripheral blood platelets, HeLa cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60237-1-Ig targets COTL1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen COTL1 fusion protein Ag1230
Full Name coactosin-like 1 (Dictyostelium)
Calculated Molecular Weight 16 kDa
Observed Molecular Weight 14-16 kDa
GenBank Accession NumberBC010884
Gene Symbol COTL1
Gene ID (NCBI) 23406
RRIDAB_2881360
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COTL1, also named as CLP, binds to F-actin in a calcium-independent manner. And it has no direct effect on actin depolymerization.

Protocols

Product Specific Protocols
WB protocol for COTL1 antibody 60237-1-IgDownload protocol
IHC protocol for COTL1 antibody 60237-1-IgDownload protocol
IF protocol for COTL1 antibody 60237-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols