CoraLite®594-conjugated CIP2A Monoclonal antibody

CIP2A Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2A3F3

Cat no : CL594-67843

Synonyms

Cancerous inhibitor of PP2A, CIP2A, FLJ12850, KIAA1524, p90, p90 autoantigen, Protein CIP2A

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Validation Data Gallery

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  IF Staining of HeLa using CL594-67843

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 CIP2A antibody (CL594-67843, Clone: 2A3F3 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67843 targets CIP2A in IF/ICC applications and shows reactivity with Human, rat samples.

• Tested Reactivity: Human, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CIP2A fusion protein Ag19598
• Full Name: KIAA1524
• Calculated Molecular Weight: 905 aa, 102 kDa
• Observed Molecular Weight: 102 kDa
• GenBank Accession Number: BC130564
• Gene Symbol: CIP2A
• Gene ID (NCBI): 57650
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Cancerous inhibitor of protein phosphatase 2A (CIP2A) was originally identified as a cellular protein phosphatase 2A (PP2A) inhibitor, and has been shown to control oncogenic cellular signals by suppressing the tumor suppressor PP2A. CIP2A overexpression has been found in several human malignancies including breast cancer, hepatocellular carcinoma, gastric cancer, head and neck cancer, colon cancer, prostate cancer and non-small cell lung cancer. CIP2A is a 83~102 kDa cytoplasmic protein. ( PMID: 26824320, PMID: 25458953, PMID: 29720672)