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CoraLite® Plus 488-conjugated CIP2A Monoclonal antibody

CIP2A Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2A3F3

Cat no : CL488-67843

Synonyms

KIAA1524, Protein CIP2A, p90 autoantigen, p90, Cancerous inhibitor of PP2A

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated CIP2A Monoclonal ant (CL488-67843)

    IF Staining of HeLa using CL488-67843

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 CIP2A antibody (CL488-67843, Clone: 2A3F3 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated CIP2A Monoclonal ant (CL488-67843)

    FC experiment of HeLa using CL488-67843

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CIP2A (CL488-67843, Clone:2A3F3) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67843 targets CIP2A in IF/ICC, FC (Intra) applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CIP2A fusion protein Ag19598
Full Name KIAA1524
Calculated Molecular Weight 905 aa, 102 kDa
Observed Molecular Weight 102 kDa
GenBank Accession NumberBC130564
Gene Symbol CIP2A
Gene ID (NCBI) 57650
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cancerous inhibitor of protein phosphatase 2A (CIP2A) was originally identified as a cellular protein phosphatase 2A (PP2A) inhibitor, and has been shown to control oncogenic cellular signals by suppressing the tumor suppressor PP2A. CIP2A overexpression has been found in several human malignancies including breast cancer, hepatocellular carcinoma, gastric cancer, head and neck cancer, colon cancer, prostate cancer and non-small cell lung cancer. CIP2A is a 83~102 kDa cytoplasmic protein. ( PMID: 26824320, PMID: 25458953, PMID: 29720672)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CIP2A antibody CL488-67843Download protocol
FC protocol for CL Plus 488 CIP2A antibody CL488-67843Download protocol
Standard Protocols
Click here to view our Standard Protocols