CIP2A Monoclonal antibody

CIP2A Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2A3F3

Cat no : 67843-1-Ig

Synonyms

Cancerous inhibitor of PP2A, CIP2A, FLJ12850, KIAA1524, p90, p90 autoantigen, Protein CIP2A

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Validation Data Gallery

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  WB analysis using 67843-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67843-1-Ig (KIAA1524 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 67843-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67843-1-Ig (KIAA1524 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 67843-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 67843-1-Ig (KIAA1524 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 67843-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using KIAA1524 antibody (67843-1-Ig, Clone: 2A3F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: LNCaP cells, HSC-T6 cells, PC-12 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67843-1-Ig targets CIP2A in WB, IF/ICC, ELISA applications and shows reactivity with Human, rat samples.

• Tested Reactivity: Human, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CIP2A fusion protein Ag19598
• Full Name: KIAA1524
• Calculated Molecular Weight: 905 aa, 102 kDa
• Observed Molecular Weight: 102 kDa
• GenBank Accession Number: BC130564
• Gene Symbol: CIP2A
• Gene ID (NCBI): 57650
• RRID: AB_2918605
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cancerous inhibitor of protein phosphatase 2A (CIP2A) was originally identified as a cellular protein phosphatase 2A (PP2A) inhibitor, and has been shown to control oncogenic cellular signals by suppressing the tumor suppressor PP2A. CIP2A overexpression has been found in several human malignancies including breast cancer, hepatocellular carcinoma, gastric cancer, head and neck cancer, colon cancer, prostate cancer and non-small cell lung cancer. CIP2A is a 83~102 kDa cytoplasmic protein. ( PMID: 26824320, PMID: 25458953, PMID: 29720672)