CoraLite® Plus 488-conjugated CHCHD4 Polyclonal antibody

• Featured Product
• KD/KO Validated

CHCHD4 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-21090

Synonyms

CHCHD4, FLJ31709, MIA40

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Validation Data Gallery

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  IF Staining of HeLa using CL488-21090

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 CHCHD4 antibody (CL488-21090) at dilution of 1:200.

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  IF Staining of A431 using CL488-21090

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 CHCHD4 antibody (CL488-21090) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: HeLa cells, A431 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-21090 targets CHCHD4 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CHCHD4 fusion protein Ag15339
• Full Name: coiled-coil-helix-coiled-coil-helix domain containing 4
• Calculated Molecular Weight: 142 aa, 16 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC033775
• Gene Symbol: CHCHD4
• Gene ID (NCBI): 131474
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CHCHD4, a component of human mitochondria, belongs to a protein family. It functions as chaperone and catalyzes the formation of disulfide bonds in substrate proteins, such as COX17. It is required for the import and folding of small cysteine-containing proteins (small Tim) in the mitochondrial intermembrane space (IMS).