CHCHD4 Polyclonal antibody

• Featured Product
• KD/KO Validated

CHCHD4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

26

Reviews (3)

Cat no : 21090-1-AP

Synonyms

MIA40, Coiled-coil-helix-coiled-coil-helix domain-containing protein 4

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Validation Data Gallery

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  WB analysis of Daudi using 21090-1-AP

  Daudi cells were subjected to SDS PAGE followed by western blot with 21090-1-AP (CHCHD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 21090-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 21090-1-AP (CHCHD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 21090-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 21090-1-AP (CHCHD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 21090-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21090-1-AP (CHCHD4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 21090-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21090-1-AP (CHCHD4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Ramos using 21090-1-AP

  Ramos cells were subjected to SDS PAGE followed by western blot with 21090-1-AP (CHCHD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 21090-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 21090-1-AP (CHCHD4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 21090-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 21090-1-AP (CHCHD4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 21090-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 21090-1-AP (CHCHD4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 21090-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 21090-1-AP (CHCHD4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 21090-1-AP

  IP result of anti-CHCHD4 (IP:21090-1-AP, 4ug; Detection:21090-1-AP 1:1000) with mouse brain tissue lysate 3200 ug.

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  IHC staining of mouse testis using 21090-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21090-1-AP (CHCHD4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 21090-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21090-1-AP (CHCHD4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 21090-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21090-1-AP (CHCHD4 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 21090-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21090-1-AP (CHCHD4 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of A431 using 21090-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CHCHD4 antibody (21090-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: Daudi cells, human heart tissue, mouse lung tissue, mouse spleen tissue, L02 cells, mouse kidney tissue, Ramos cells, Raji cells, rat brain tissue, mouse brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse testis tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 24 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

21090-1-AP targets CHCHD4 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CHCHD4 fusion protein Ag15339
• Full Name: coiled-coil-helix-coiled-coil-helix domain containing 4
• Calculated Molecular Weight: 142 aa, 16 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC033775
• Gene Symbol: CHCHD4
• Gene ID (NCBI): 131474
• RRID: AB_10734583
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CHCHD4, a component of human mitochondria, belongs to a protein family. It functions as chaperone and catalyzes the formation of disulfide bonds in substrate proteins, such as COX17. It is required for the import and folding of small cysteine-containing proteins (small Tim) in the mitochondrial intermembrane space (IMS).

Publications

Species	Application	Title
human	WB	Cell Stem Cell; Disrupting Mitochondrial Copper Distribution Inhibits Leukemic Stem Cell Self-Renewal.; Authors - Rashim Pal Singh
rat	WB,IF	Exp Mol Med; SIRT5-related desuccinylation modification of AIFM1 protects against compression-induced intervertebral disc degeneration by regulating mitochondrial homeostasis; Authors - Jianxin Mao
human	WB,IF	Aging Cell; Dependence of PINK1 accumulation on mitochondrial redox system.; Authors - Feng Gao ; KD Validated
human	WB	Redox Biol; Flow-induced endothelial mitochondrial remodeling mitigates mitochondrial reactive oxygen species production and promotes mitochondrial DNA integrity in a p53-dependent manner.; Authors - Junchul Shin
human	WB	EMBO Mol Med; A novel CHCHD10 mutation implicates a Mia40-dependent mitochondrial import deficit in ALS.; Authors - Carina Lehmer
human	WB	Oncogene; CHTM1, a novel metabolic marker deregulated in human malignancies.; Authors - Mansi Babbar

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Veronica (Verified Customer) (10-17-2023)

First time I find an anti-CHCHD4 antibody that works fine. We are testing it in IF as well, but till now I am already really happy with the result in WB. No aspecific signal, clear band. Reccomended! Conditions: Gradient gel 8-15% Ab’: 1:1000 in 5% milk O/N

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T; HeLa; isolated mitochondria from HEK293T

CHCHD4 Antibody Western Blot validation (1:1000 dilution) in HEK293T; HeLa; isolated mitochondria from HEK293T (Cat no:21090-1-AP)

FH

Süleyman (Verified Customer) (01-23-2022)

CHCHD4 antibody (Green) is used 1:100 dilution (2 hour at RT) and 1:700 of Alexa Fluor 488 secondary antibody (1 hour RT). 4% Paraformaldehyde fixation for 10 min at RT. Permeabilization with 0.3% Triton X for 10-15 minutes at RT. 5% FBS in PBS used for blocking for 1 hour at RT. The image is colocalized with TOMM20 (Red). Blue is DAPI. As you can see there is colocalization with TOMM20 as CHCHD4 supposed to be in IMS but I also see unspecific bindings in cytosolic proteins. Therefore my rating is 3 as it might be binding unspecifically.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: HeLa FlipIn

CHCHD4 Antibody Immunofluorescence validation (1:100 dilution) in HeLa FlipIn (Cat no:21090-1-AP)

FH

Lana (Verified Customer) (08-13-2019)

SDS-PAGE: 10 ug/ul RIPA lysate of the mitochondrial fraction of HEK293t. 4-12% Bis-tris gradient gel.Transfer: Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4CBlocking: SEA Block Blocking Buffer 1hPrimary Ab: O/N incubation at 4CSecondary Ab: IRDye 800CW Goat anti-Rabbit

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293t

CHCHD4 Antibody Western Blot, validation (1:1000 dilution) in HEK293t (Cat no:21090-1-AP)