CEP250/CNAP1 Polyclonal antibody

CEP250/CNAP1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human and More (1)





Cat no : 14498-1-AP


250 kDa centrosomal protein, C NAP1, Centrosomal protein 2, centrosomal protein 250kDa, CEP2, CEP250, CEP250/CNAP1, CNAP1, C-NAP1

Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhTERT-RPE1 cells, HeLa cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14498-1-AP targets CEP250/CNAP1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CEP250/CNAP1 fusion protein Ag5925
Full Name centrosomal protein 250kDa
Calculated Molecular Weight 281 kDa
Observed Molecular Weight 250 kDa
GenBank Accession NumberBC001433
Gene Symbol CEP250
Gene ID (NCBI) 11190
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CEP250, also known as C-Nap1, is a 250 kDa coiled-coil protein that localizes to the proximal ends of mother and daughter centrioles. It is required for centriole-centriole cohesion during interphase of the cell cycle. It dissociates from the centrosomes when parental centrioles separate at the beginning of mitosis. The protein associates with and is phosphorylated by NIMA-related kinase 2, which is also associated with the centrosome.


Product Specific Protocols
WB protocol for CEP250/CNAP1 antibody 14498-1-APDownload protocol
IHC protocol for CEP250/CNAP1 antibody 14498-1-APDownload protocol
IF protocol for CEP250/CNAP1 antibody 14498-1-APDownload protocol
IP protocol for CEP250/CNAP1 antibody 14498-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes.

Authors - Chun So

Nat Commun

Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis.

Authors - Hai-Qing Tu

Nat Commun

Cytoplasmic E2f4 forms organizing centres for initiation of centriole amplification during multiciliogenesis.

Authors - Munemasa Mori

PLoS Biol

Stable centrosomal roots disentangle to allow interphase centriole independence.

Authors - Robert Mahen

Proc Natl Acad Sci U S A

Dishevelled is a NEK2 kinase substrate controlling dynamics of centrosomal linker proteins.

Authors - Igor Cervenka

J Cell Biol

Uncoordinated centrosome cycle underlies the instability of non-diploid somatic cells in mammals.

Authors - Kan Yaguchi


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Karsten (Verified Customer) (12-22-2022)

WB: 1:600 dilution in 5% Milch in 1xTBST over nigth at 4°C, 30µg Protein loaded: result show prominent band at a hight of 250 kDa. unspecific bands also visible, but not prominent. worked fine IFS: 1:100, MetOH (-20°) fixation for 5 min at RT , Permeabilisation with 0.3% PBST (Triton) for 5 min at RT, Blocking with 1% BSA in PBS for 1h at RT. Antibody dilution 1:100 in 1%BSA in PBS 1h RT, sek Ak 1h at RT- nice basal body stainings at cilia in hTERT-RPE1 cells, that were starved for 3 days.

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:600
  • Cell Tissue Type: hTERT-RPE1 cells, 3 days starved
CEP250/CNAP1 Antibody Western Blot,Immunofluorescence validation (1:600 dilution) in hTERT-RPE1 cells, 3 days starved (Cat no:14498-1-AP)

Hairuo (Verified Customer) (12-16-2019)

Used in IF in neonatal mouse testis cry-sections.Works well.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:500