CEP250/CNAP1 Polyclonal antibody

CEP250/CNAP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

34

Reviews (2)

Cat no : 14498-1-AP

Synonyms

Cep250, CEP250,C-NAP1, Centrosome-associated protein CEP250, centrosomal protein 250kDa, Centrosomal protein 2

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Validation Data Gallery

• 

  WB analysis of HeLa using 14498-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14498-1-AP (CEP250/CNAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14498-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14498-1-AP (CEP250/CNAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 14498-1-AP

  IP result of anti-CEP250/CNAP1 (IP:14498-1-AP, 5ug; Detection:14498-1-AP 1:500) with HeLa cells lysate 1200ug.

• 

  IHC staining of human placenta using 14498-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14498-1-AP (CEP250/CNAP1 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human placenta using 14498-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14498-1-AP (CEP250/CNAP1 antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of hTERT-RPE1 using 14498-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using CEP250/CNAP1 antibody (14498-1-AP) at dilution of 1:100 and Cy3-conjugated Goat Anti-Rabbit IgG(H+L), SCLT1 antibody (14875-1-AP, Magenta), CoraLite®488 ARL13B antibody (CL488-17711, green).

• 

  IF Staining of HeLa using 14498-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14498-1-AP (CEP250/CNAP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: hTERT-RPE1 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 30 publications below

Product Information

14498-1-AP targets CEP250/CNAP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CEP250/CNAP1 fusion protein Ag5925
• Full Name: centrosomal protein 250kDa
• Calculated Molecular Weight: 281 kDa
• Observed Molecular Weight: 250 kDa
• GenBank Accession Number: BC001433
• Gene Symbol: CEP250/CNAP1
• Gene ID (NCBI): 11190
• RRID: AB_2076918
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CEP250, also known as C-Nap1, is a 250 kDa coiled-coil protein that localizes to the proximal ends of mother and daughter centrioles. It is required for centriole-centriole cohesion during interphase of the cell cycle. It dissociates from the centrosomes when parental centrioles separate at the beginning of mitosis. The protein associates with and is phosphorylated by NIMA-related kinase 2, which is also associated with the centrosome.

Publications

Species	Application	Title
mouse	IF	Science; A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes.; Authors - Chun So
human	IF	Nat Commun; Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis.; Authors - Hai-Qing Tu
mouse	IF	Nat Commun; Cytoplasmic E2f4 forms organizing centres for initiation of centriole amplification during multiciliogenesis.; Authors - Munemasa Mori
human	IF	PLoS Biol; Stable centrosomal roots disentangle to allow interphase centriole independence.; Authors - Robert Mahen
human	WB,IF	Proc Natl Acad Sci U S A; Dishevelled is a NEK2 kinase substrate controlling dynamics of centrosomal linker proteins.; Authors - Igor Cervenka
	IF	J Cell Biol; Uncoordinated centrosome cycle underlies the instability of non-diploid somatic cells in mammals.; Authors - Kan Yaguchi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Karsten (Verified Customer) (12-22-2022)

WB: 1:600 dilution in 5% Milch in 1xTBST over nigth at 4°C, 30µg Protein loaded: result show prominent band at a hight of 250 kDa. unspecific bands also visible, but not prominent. worked fine IFS: 1:100, MetOH (-20°) fixation for 5 min at RT , Permeabilisation with 0.3% PBST (Triton) for 5 min at RT, Blocking with 1% BSA in PBS for 1h at RT. Antibody dilution 1:100 in 1%BSA in PBS 1h RT, sek Ak 1h at RT- nice basal body stainings at cilia in hTERT-RPE1 cells, that were starved for 3 days.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:600
• Cell Tissue Type: hTERT-RPE1 cells, 3 days starved

CEP250/CNAP1 Antibody Western Blot,Immunofluorescence validation (1:600 dilution) in hTERT-RPE1 cells, 3 days starved (Cat no:14498-1-AP)

FH

Hairuo (Verified Customer) (12-16-2019)

Used in IF in neonatal mouse testis cry-sections.Works well.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500

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