CoraLite® Plus 488-conjugated CEP250/CNAP1 Polyclonal antibody

CEP250/CNAP1 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-14498

Synonyms

250 kDa centrosomal protein, C NAP1, Centrosomal protein 2, centrosomal protein 250kDa, CEP2, CEP250, CNAP1

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Validation Data Gallery

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  IF Staining of HeLa using CL488-14498

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 CEP250/CNAP1 antibody (CL488-14498) at dilution of 1:100, CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).

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  IF Staining of MCF-7 using CL488-14498

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using CoraLite® Plus 488 CEP250/CNAP1 antibody (CL488-14498) at dilution of 1:100.

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Tested Applications

• Positive IF detected in: HeLa cells, MCF-7 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-14498 targets CEP250/CNAP1 in IF applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CEP250/CNAP1 fusion protein Ag5925
• Full Name: centrosomal protein 250kDa
• Calculated Molecular Weight: 281 kDa
• Observed Molecular Weight: 250 kDa
• GenBank Accession Number: BC001433
• Gene Symbol: CEP250
• Gene ID (NCBI): 11190
• RRID: AB_2919094
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CEP250, also known as C-Nap1, is a 250 kDa coiled-coil protein that localizes to the proximal ends of mother and daughter centrioles. It is required for centriole-centriole cohesion during interphase of the cell cycle. It dissociates from the centrosomes when parental centrioles separate at the beginning of mitosis. The protein associates with and is phosphorylated by NIMA-related kinase 2, which is also associated with the centrosome.