CEA/CD66e Recombinant antibody, PBS Only

• 

  WB analysis of MCF-7 using 83653-5-RR (same clone as 83653-5-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 83653-5-RR (CEA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83653-5-PBS in a different storage buffer formulation.

• 

  WB analysis of human saliva using 83653-5-RR (same clone as 83653-5-PBS)

  human saliva were subjected to SDS PAGE followed by western blot with 83653-5-RR (CEA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83653-5-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary cancer using 83653-5-RR (same clone as 83653-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83653-5-RR (CEA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83653-5-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary cancer using 83653-5-RR (same clone as 83653-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83653-5-RR (CEA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83653-5-PBS in a different storage buffer formulation.

• 

  IF Staining of HT-29 using 83653-5-RR (same clone as 83653-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using CEA antibody (83653-5-RR, Clone: 240666B12 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83653-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83653-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83653-5-RR against Human CEA were performed. The affinity constant is 0.734 nM.

CEA/CD66e Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 83653-5-PBS

Clone No.240666B12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CEA, CEACAM5, CD66e, Carcinoembryonic antigen-related cell adhesion molecule 5, Carcinoembryonic antigen

---

Product Information

83653-5-PBS targets CEA/CD66e in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: carcinoembryonic antigen-related cell adhesion molecule 5
• Calculated Molecular Weight: 77 kDa
• Observed Molecular Weight: 200 kDa
• GenBank Accession Number: NM_004363
• Gene Symbol: CEA
• Gene ID (NCBI): 1048
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P06731-1
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Carcinoembryonic antigen (CEA), also known as CEACAM5 or CD66e, is a cell surface glycoprotein belonging to the immunoglobulin superfamily, mainly serving as a cell adhesion molecule mediating intercellular contact by both homophilic and heterophilic binding (PMID: 21731662). CEA inhibits anoikis and plays a role in tumorigenesis and metastasis. CEA has been found to be overexpressed in a wide variety of human cancers, including colon, breast, and lung (PMID: 17167768). CEA is a tumor marker and is routinely exploited for diagnosis. This antibody is raised against 35-685 aa of human CEA/CD66e and can cross-react with CEACAM1/CD66a and CEACAM3/CD66d.