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CEA/CD66e Recombinant antibody

CEA/CD66e Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240666B12

Cat no : 83653-5-RR

Synonyms

CEA, CEACAM5, CD66e, Carcinoembryonic antigen-related cell adhesion molecule 5, Carcinoembryonic antigen

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using CEA Recombinant antibody (83653-5-RR)

    WB analysis of MCF-7 using 83653-5-RR

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 83653-5-RR (CEA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human saliva using CEA Recombinant antibody (83653-5-RR)

    WB analysis of human saliva using 83653-5-RR

    human saliva were subjected to SDS PAGE followed by western blot with 83653-5-RR (CEA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using CEA Recombinant antibody (83653-5-RR)

    IHC staining of human ovary cancer using 83653-5-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83653-5-RR (CEA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using CEA Recombinant antibody (83653-5-RR)

    IHC staining of human ovary cancer using 83653-5-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83653-5-RR (CEA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HT-29 cells using CEA Recombinant antibody (83653-5-RR)

    IF Staining of HT-29 using 83653-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using CEA antibody (83653-5-RR, Clone: 240666B12 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83653-5-RR

    Affinity and Kinetic Characterization of 83653-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83653-5-RR against Human CEA were performed. The affinity constant is 0.734 nM.

Tested Applications

Positive WB detected inMCF-7 cells, human saliva
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHT-29 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83653-5-RR is CEA/CD66e Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name carcinoembryonic antigen-related cell adhesion molecule 5
Calculated Molecular Weight 77 kDa
Observed Molecular Weight200 kDa
GenBank Accession NumberNM_004363
Gene Symbol CEA
Gene ID (NCBI) 1048
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Carcinoembryonic antigen (CEA), also known as CEACAM5 or CD66e, is a cell surface glycoprotein belonging to the immunoglobulin superfamily, mainly serving as a cell adhesion molecule mediating intercellular contact by both homophilic and heterophilic binding (PMID: 21731662). CEA inhibits anoikis and plays a role in tumorigenesis and metastasis. CEA has been found to be overexpressed in a wide variety of human cancers, including colon, breast, and lung (PMID: 17167768). CEA is a tumor marker and is routinely exploited for diagnosis.

Protocols

Product Specific Protocols
WB protocol for CEA/CD66e antibody 83653-5-RRDownload protocol
IHC protocol for CEA/CD66e antibody 83653-5-RRDownload protocol
IF protocol for CEA/CD66e antibody 83653-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols