CEA/CD66e Recombinant antibody
CEA/CD66e Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
240666B12
Cat no : 83653-5-RR
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | MCF-7 cells, human saliva |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HT-29 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83653-5-RR targets CEA/CD66e in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Fusion Protein |
| Full Name | carcinoembryonic antigen-related cell adhesion molecule 5 |
| Calculated Molecular Weight | 77 kDa |
| Observed Molecular Weight | 200 kDa |
| GenBank Accession Number | NM_004363 |
| Gene Symbol | CEA |
| Gene ID (NCBI) | 1048 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Carcinoembryonic antigen (CEA), also known as CEACAM5 or CD66e, is a cell surface glycoprotein belonging to the immunoglobulin superfamily, mainly serving as a cell adhesion molecule mediating intercellular contact by both homophilic and heterophilic binding (PMID: 21731662). CEA inhibits anoikis and plays a role in tumorigenesis and metastasis. CEA has been found to be overexpressed in a wide variety of human cancers, including colon, breast, and lung (PMID: 17167768). CEA is a tumor marker and is routinely exploited for diagnosis. This antibody is raised against 35-685 aa of human CEA/CD66e and can cross-react with CEACAM1/CD66a and CEACAM3/CD66d.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CEA/CD66e antibody 83653-5-RR | Download protocol |
| IHC protocol for CEA/CD66e antibody 83653-5-RR | Download protocol |
| IF protocol for CEA/CD66e antibody 83653-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Jan (Verified Customer) (01-06-2025) | Immunofluorescence analysis of CEA in BxPC-3 cells. Cells were incubated with CEA/CD66e Recombinant antibody (83653-5-RR) and TRITC-conjugated Goat Anti-Rabbit IgG(H+L) (SA00007-2).
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