CoraLite® Plus 488-conjugated CDX2 Recombinant antibody

CDX2 Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3J1

Cat no : CL488-82659

Synonyms

CDX-2, CDX 3, Caudal-type homeobox protein 2, Caudal type homeobox protein 2, Caudal type homeobox 2

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Validation Data Gallery

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  IF Staining of Caco-2 using CL488-82659

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using CoraLite® Plus 488 CDX2 antibody (CL488-82659, Clone: 3J1 ) at dilution of 1:400.

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  IF Staining of Caco-2 using CL488-82659

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using CoraLite® Plus 488 CDX2 antibody (CL488-82659, Clone: 3J1 ) at dilution of 1:200, Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

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  FC experiment of Caco-2 using CL488-82659

  1x10^6 Caco-2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated CDX2 Recombinant antibody (CL488-82659, Clone:3J1)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive IF/ICC detected in: Caco-2 cells
• Positive FC (Intra) detected in: Caco-2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-82659 targets CDX2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CDX2 fusion protein Ag17310
• Full Name: caudal type homeobox 2
• Calculated Molecular Weight: 313 aa, 34 kDa
• Observed Molecular Weight: 33-40 kDa
• GenBank Accession Number: BC014461
• Gene Symbol: CDX2
• Gene ID (NCBI): 1045
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CDX2, also named as Homeobox protein CDX-2, is a 313 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the Caudal homeobox family. CDX2 localizes in the nucleus and is involved in the transcriptional regulation of multiple genes expression in the intestinal epithelium. The relative expression of CDX1 to CDX2 protein may be important in the anterior to posterior patterning of the intestinal epithelium and in defining patterns of proliferation and differentiation along the crypt-villus axis. Both Cdx1 and Cdx2 genes must be expressed to reduce tumorigenic potential, to increase sensitivity to apoptosis, and to reduce cell migration, suggesting that the two genes control the normal phenotype by independent pathways.