CDX2 Recombinant antibody

CDX2 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

3J1

Cat no : 82659-1-RR

Synonyms

CDX-2, CDX 3, Caudal-type homeobox protein 2, Caudal type homeobox protein 2, Caudal type homeobox 2

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Validation Data Gallery

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  WB analysis using 82659-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82659-1-RR (CDX2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 82659-1-RR

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 82659-1-RR (CDX2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IHC staining of rat colon using 82659-1-RR

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 82659-1-RR (CDX2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 82659-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 82659-1-RR (CDX2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 82659-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 82659-1-RR (CDX2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Caco-2 using 82659-1-RR

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using CDX2 antibody (82659-1-RR, Clone: 3J1 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

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  Affinity and Kinetic Characterization of 82659-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82659-1-RR against Human CDX2 were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: Caco-2 cells, mouse colon tissue, COLO 320 cells
• Positive IHC detected in: rat colon tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Caco-2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

82659-1-RR targets CDX2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CDX2 fusion protein Ag17310
• Full Name: caudal type homeobox 2
• Calculated Molecular Weight: 313 aa, 34 kDa
• Observed Molecular Weight: 33-40 kDa
• GenBank Accession Number: BC014461
• Gene Symbol: CDX2
• Gene ID (NCBI): 1045
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDX2, also named as Homeobox protein CDX-2, is a 313 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the Caudal homeobox family. CDX2 localizes in the nucleus and is involved in the transcriptional regulation of multiple genes expression in the intestinal epithelium. The relative expression of CDX1 to CDX2 protein may be important in the anterior to posterior patterning of the intestinal epithelium and in defining patterns of proliferation and differentiation along the crypt-villus axis. Both Cdx1 and Cdx2 genes must be expressed to reduce tumorigenic potential, to increase sensitivity to apoptosis, and to reduce cell migration, suggesting that the two genes control the normal phenotype by independent pathways.

Publications

Species	Application	Title
human	IF	Biofabrication; 3D bioprinting platform development for high-throughput cancer organoid models construction and drug evaluation; Authors - Rui Dai