CoraLite® Plus 488-conjugated CDC2L1 Polyclonal antibody

• Featured Product
• KD/KO Validated

CDC2L1 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-28613

Synonyms

CDK11, CDK11B, CDK11 p58, CDK11 p46, CDK11 p110

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Validation Data Gallery

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  IF Staining of HUVEC using CL488-28613

  Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using CoraLite® Plus 488 CDC2L1 antibody (CL488-28613) at dilution of 1:200, CL594-phalloidin (red).

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  FC experiment of HeLa using CL488-28613

  1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated CDC2L1 Polyclonal antibody (CL488-28613)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive IF/ICC detected in: HUVEC cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-28613 targets CDC2L1 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CDC2L1 fusion protein Ag28743
• Full Name: cell division cycle 2-like 1 (PITSLRE proteins)
• Calculated Molecular Weight: 93 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: NM_001787
• Gene Symbol: CDC2L1
• Gene ID (NCBI): 984
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CDK11 is encoded by two highly homologous genes known as CDC2L1 and CDC2L2. Due to the distinct structure and alterative RNA splicing, the CDC2L gene can produce three different CDK11 isoforms, a larger 110 kDa protein isoform, a mitosis-specific 58 kDa isoform, and a smaller apoptosis-specific 46 kDa isoform.(PMID 27049727)