Biotin-conjugated CD81 Monoclonal antibody

CD81 Monoclonal Antibody for IHC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IHC

Conjugate

Biotin

CloneNo.

1G2C6

Cat no : Biotin-66866

Synonyms

CD81, CD81 antigen, CD81 molecule, S5.7, TAPA1, Tetraspanin 28, Tspan 28, TSPAN28

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Validation Data Gallery

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  IHC staining of human liver using Biotin-66866

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using Biotin-66866 (CD81 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using Biotin-66866

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using Biotin-66866 (CD81 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:100-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-66866 targets CD81 in IHC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD81 fusion protein Ag27298
• Full Name: CD81 molecule
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC002978
• Gene Symbol: CD81
• Gene ID (NCBI): 975
• Conjugate: Biotin
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CD81 (also known as TAPA1 or TSPAN28) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD81 is involved in signal transduction and cell adhesion in the immune system (PMID: 9597125). CD81 has also been identified as en essential receptor for HCV (hepatitis C virus) (PMID: 21428934).