CD81 Monoclonal antibody

CD81 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human and More (2)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

149

CloneNo.

1G2C6

Reviews (1)

Cat no : 66866-1-Ig

Synonyms

TAPA1, S5.7, CD81 molecule, CD81 antigen, 26 kDa cell surface protein TAPA-1

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Validation Data Gallery

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  WB analysis using 66866-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66866-1-Ig (CD81 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66866-1-Ig

  HEK-293 cells and HEK-293-derived exosomes (HEK-293-exo) were subjected to SDS PAGE followed by western blot with 66866-1-Ig (CD81 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 66866-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66866-1-Ig (CD81 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of THP-1 using 66866-1-Ig

  LPS treated THP-1 cells were subjected to SDS PAGE followed by western blot with 66866-1-Ig (CD81 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 66866-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66866-1-Ig (CD81 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 66866-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66866-1-Ig (CD81 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human tonsillitis using 66866-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD81 antibody (66866-1-Ig, Clone: 1G2C6 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human tonsillitis using 66866-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD81 antibody (66866-1-Ig, Clone: 1G2C6 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: Raji cells, Jurkat cells, LPS treated THP-1 cells, Ramos cells, Daudi cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human tonsillitis tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:600-1:2400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 145 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• FC: See 4 publications below

Product Information

66866-1-Ig targets CD81 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD81 fusion protein Ag27298
• Full Name: CD81 molecule
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC002978
• Gene Symbol: CD81
• Gene ID (NCBI): 975
• RRID: AB_2882203
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD81 (also known as TAPA1 or TSPAN28) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD81 is involved in signal transduction and cell adhesion in the immune system (PMID: 9597125). CD81 has also been identified as en essential receptor for HCV (hepatitis C virus) (PMID: 21428934).

Publications

Species	Application	Title
human	WB	Gastroenterology; PTEN deficiency facilitates exosome secretion and metastasis in cholangiocarcinoma by impairing TFEB-mediated lysosome biogenesis; Authors - Tian-Yi Jiang
human	WB	J Extracell Vesicles; Extracellular vesicles from lung tissue drive bone marrow neutrophil recruitment in inflammation.; Authors - Bowen Liu
	WB	Sci Adv; Avian ANP32A incorporated in avian influenza A virions promotes interspecies transmission by priming early viral replication in mammals; Authors - Lei Na
human	WB	J Exp Clin Cancer Res; Circular RNA circATP9A promotes non-small cell lung cancer progression by interacting with HuR and by promoting extracellular vesicles-mediated macrophage M2 polarization; Authors - Yuanshan Yao
human	WB,IF	J Exp Clin Cancer Res; Chemotherapy-elicited extracellular vesicle CXCL1 from dying cells promotes triple-negative breast cancer metastasis by activating TAM/PD-L1 signaling; Authors - Shengqi Wang
human	WB	Drug Des Devel Ther; Keloid Patient Plasma-Derived Exosomal hsa_circ_0020792 Promotes Normal Skin Fibroblasts Proliferation, Migration, and Fibrogenesis via Modulating miR-193a-5p and Activating TGF-β1/Smad2/3 Signaling; Authors - Huan Hu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Xiaogang (Verified Customer) (10-21-2021)

I recently bought this CD81 antibody to detect CD81 from EVs purified from human and mouse cell lines. The antibody works well to detect CD81 by immunoblot.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: RAW264.7 and differentiated THP-1 cells

CD81 Antibody Western Blot validation (1:1000 dilution) in RAW264.7 and differentiated THP-1 cells (Cat no:66866-1-Ig)