CoraLite® Plus 488-conjugated CD81 Monoclonal antibody

CD81 Monoclonal Antibody for IF, FC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF, FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G2C6

Cat no : CL488-66866

Synonyms

CD81, CD81 antigen, CD81 molecule, Exosome marker, S5.7, TAPA1, Tetraspanin 28, Tspan 28, TSPAN28



Tested Applications

Positive IF detected inhuman tonsillitis tissue
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66866 targets CD81 in IF, FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD81 fusion protein Ag27298
Full Name CD81 molecule
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC002978
Gene Symbol CD81
Gene ID (NCBI) 975
RRIDAB_2919381
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

CD81 (also known as TAPA1 or TSPAN28) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD81 is involved in signal transduction and cell adhesion in the immune system (PMID: 9597125). CD81 has also been identified as en essential receptor for HCV (hepatitis C virus) (PMID: 21428934).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CD81 antibody CL488-66866Download protocol
Standard Protocols
Click here to view our Standard Protocols